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Environmental fate & pathways

Adsorption / desorption

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Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
August 25 to September 5, 2009
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2-Octyldodecan-1-ol
- Substance type: pur active substance
- Physical state: liquid
- Lot/batch No.: 03508
- Expiration date of the lot/batch: January 1, 2011
- Storage condition of test material: room temperature, protected from moisture and light
Radiolabelling:
no

Study design

Test temperature:
Column temperature: 20 °C
Temperature (RI): 30 °C

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HPLC
- Type: Waters 616 with autosampler Waters 717 plus
- Type, material and dimension of analytical (guard) column: YMC-Pack Cyano, cyanopropyl solid phase, 250 x 4.0 mm, YMC Europe GmbH
- Detection system: 2996 Diode Array Detector (DAD), Waters, for the reference items and IR detector HP 1047 A, Hewlett Packard, for the test item

MOBILE PHASES
- Type: 70:30 (% v/v) acetonritrile : HPLC water
- Experiments with additives carried out on separate columns: no
- Solutes for dissolving test and reference substances: acteonitril/water for test substance, methanol for reference substances


DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column: NaNO3


REFERENCE SUBSTANCES
- Identity:
Acetanilide (CAS 103-84-4), Atrazine (CAS 1912-24-9), Monuron (CAS 150-68-5), 2,5-Dichloroaniline (CAS 95-82-9), Benzoic acid phenylester (CAS 93-99-2), Fenthion (CAS 55-38-9), Phenanthrene (CAS 85-01-8), 4,4¿-DDT (CAS 50-29-3)


DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 100 µl of 400 mg/l solution
- Quantity of reference substances: 100 µl of 100 mg/l solution
- Intervals of calibration: each reference item was analysed separately before and after analysis of the test item


REPETITIONS
- Number of determinations: 3


EVALUATION
- Calculation of capacity factors k': k'= (retention time tR - dead time t0)/dead time t0
- Determination of the log Koc value: By means of the adsorption coefficient values (Koc) and the corresponding capacity factors of the reference items a calibration calibration graph of log k` vs. log Koc,soil and log k ' versus logKoc, sewage sludge was plotted. The Koc values of the test item was then calculated based on the calibration curves.

Results and discussion

Adsorption coefficientopen allclose all
Key result
Type:
Koc
Value:
ca. 840 000 000
Key result
Type:
log Koc
Value:
ca. 8.92

Results: HPLC method

Details on results (HPLC method):
- Retention times of reference substances used for calibration [min]:
Acetanilide: 3.333
Atrazine: 3.396
Monuron: 3.428
2,5-Dichloroaniline: 3.476
Benzoic acid phenylester: 3.513
Fenthion: 3.558
Phenanthrene: 3.669
4,4'-DDT: 3.847
- Details of fitted regression line (log k' vs. log Koc): For soil the slope of the regression line were determined 0.0282 with an intercept of -0.0892. For sewage sludge the slope of the regression line were determined 0.0256 with an intercept of -0.0876.
- Average retention data for test substance [min]: 4.354

Any other information on results incl. tables

log Koc, soil = 8.92, Koc, soil=8.4x10exp8

log Koc, sewage sludge = 9.79, Koc, sewage sludge=6.2x10exp9.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
For 2-Octyldodecanol for soil the log Koc was determined to be 8.92 with a corresponding KOC value of 8.4x10exp8. For sewage sludge the log Koc was determined to be 9.79 with a corresponding Koc of 6.2x10exp9.
Executive summary:

At test conditions the retention time of the test substance 2 -Octyldodecan-1 -ol was found to be higher as the retention time of the reference item 4,4-DDT (highest Koc value in the set). Therefore the log Koc values were extrapolated.

For 2-Octyldodecanol for soil the log Koc was determined to be 8.92 with a corresponding KOC value of 8.4x10exp8. For sewage sludge the log Koc was determined to be 9.79 with a corresponding Koc of 6.2x10exp9.