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EC number: 700-073-5 | CAS number: 1215036-04-6
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 29/01/09 to 30/03/09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study conducted under good laboratory practices
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Isosorbide Diesters
- EC Number:
- 700-073-5
- Cas Number:
- 1215036-04-6
- Molecular formula:
- As it is an UVCB, we can not provide the exact molecular formula.
- IUPAC Name:
- Isosorbide Diesters
- Details on test material:
- - Name of test material (as cited in study report): LAB 3822
- Physical state: LIQUID
- Lot/batch No.: FAL 07/25
- Expiration date of the lot/batch: March 2009
- Storage condition of test material:at room temperature but preferably between 10 and 15°C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The LAB 3822 is slightly soluble in water (≤ 5mg/l). In order to test the dissolved part of the test item, a stock solution at 1000 mg/L was prepared, for each renewal, in dilution water as follows: 1/ Ultrasonication of the test item (1 g) during 10 minutes in about 500 mL of dilution water 2/ 450 mL are collected in a 1 liter flask 3/ Ultrasonication of the 50 mL residual solution during 2 minutes in about 100 mL of dilution water 4/ 150 mL are collected in the 1 liter flask and up to 1 liter with dilution water 5/ Magnetic stirring during 10 minutes 6/ Centrifugation for 30 minutes (3000 g).The test solution was renewed every 24 hours.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Name : Zebrafish Danio rerio
- Source: Elevage de la Grande Rivière (69490 Saint Forgeux, France)
- Length at study initiation (length definition, mean, range and SD):Mean size: 27.5 +/- 1.6 mm
- Weight at study initiation (mean and range, SD): 0.37g +/-0.06g
- Food type: TetraMin Tetra
ACCLIMATION
- Acclimation period:62 days
- Acclimation conditions (same as test or not):same as test
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 240 mg/L expressed as CaCO3
- Test temperature:
- 23°C +/- 1°C in a temperature-controlled room
- pH:
- 7.9
- Dissolved oxygen:
- 8.9 mg/l
- Details on test conditions:
- Vessel: 5 litres glass vessels filled with 4 litres
Test: 1 blank control + 1 test vessel
Number of animals: 7 fish in 4 litres
Test concentration: 100 mg/L (initial nominal concentration)
Biomass: 0.65 g/L
Feeding: none
Light: 14-hour light / 10-hour dark cycle
Temperature: 23°C 1°C in a temperature-controlled room
Aeration: none
Renewal: every 24 hours (test concentration and control)
Duration: 96 hours
Times of observation: 6 - 24 – 48 – 72 – 96 hours.
Toxic effect: Fish are considered dead if there is no visible movement (e.g. gill movements) and if touching of the caudal peduncle produces no reaction. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No lethal effect was observed in this test for the LAB 3822 at the nominal concentration of 100 mg/L after 96 hours.
- Executive summary:
TEST PURPOSE
Determination, under defined conditions, of the lethal effects of the test item on a test population of Danio rerio.
GUIDELINE OECD
guideline for testing of chemicals No 203: Fish, Acute Toxicity Test (Adopted 17 July 1992).
TEST SYSTEM
Zebrafish Danio rerio
TEST ITEM LAB 3822
TEST CONCENTRATION 100 mg/L (initial nominal concentration).
DURATION 96 hours (semi-static conditions, renewal every 24 hours).
Mortality observed within the test period
Exposure time Control LAB 3822100mg/L 6h 0 0 24h 0 0 48h 0 0 72h 0 0 96h 0 0 Mortality after 96h (%) 0% 0% RESULTS
No lethal effect was observed in this test for the LAB 3822 at the nominal concentration of 100 mg/L after 96 hours.
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