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EC number: 250-437-8 | CAS number: 31024-56-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the analogue approach justification provided in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Conclusions:
- A 96-h LC50 >100 mg/l in Danio rerio was obtained in a guideline test and in accordance with GLP. The result is taken to be reliable.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 July - 06 July 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 and Nominal 100 mg/L
- Sampling method: Verification of test item concentrations was performed by analysis of silicon at the start and the end of the test.
Duplicate samples were taken from the approximate centre of the aquaria, without stiring, from the freshly prepared test medium of the single test concentration and from the control, just before the start of the test. Additional duplicate samples were taken from the test medium and the control after 48 hours and at the end of the test (96 hours).
- Sample storage conditions before analysis: Samples were immediately deep frozen (at about -20 °C) after sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the only test concentration of nominal 100 mg/L was freshly prepared by dissolving 501 mg of the test item completely in to 5000 ml test water by stiring for 5 mins at room temperature.
The pH of test medium was 9.4 therefore the pH was adjusted to pH 7.8 using a concentrated hydrochloric acid solution (2 M).
- Eluate:
- Differential loading:
- Controls: Test water without test medium
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Not stated - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Strain: Not stated
- Source: Zoohaus Schaub, CH-4410 Liestal, Switzerland
- Age at study initiation (mean and range, SD): Not stated
- Length at study initiation (length definition, mean, range and SD): 3.5 ± 0.2 cm (mean ± SD)
- Weight at study initiation (mean and range, SD): 0.4 ± 0.10 g (mean ± SD)
- Feeding during test: No
ACCLIMATION
- Acclimation period: 1 week in test water at test temperature
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Fed until one day before the start of the test with a commercial fish diet (TETRA MIN Hauptfutter, supplied by TETRA-Werke, D-49304 Melle, Germany). Amount not stated.
- Health during acclimation (any mortality observed): < 5 % mortality and all fish were healthy - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 2.5 mmol/l test water
- Test temperature:
- 22-23 °C
- pH:
- 7.8 - 8.0
- Dissolved oxygen:
- 7.8-8.2 mg/L
- Nominal and measured concentrations:
- 0 mg/L control and Nominal 100 mg/L
Measured and corrected by mean recovery of spiked samples (from Nominal concentration of 100 mg/L): mean of 115 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquarium
- Type (delete if not applicable): open / closed: Not stated
- Material, size, headspace, fill volume: One glass auarium with 5 litres of test medium for each treatment
- Aeration: Test water was aerated until oxygen saturation was reached anded during the test period slightly aerat
- Type of flow-through (e.g. peristaltic or proportional diluter): Not stated
- Renewal rate of test solution (frequency/flow rate): No renewal
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.56 g fish wet weight per litre test medium
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water. Analytical grade salts were dissolved in deionized water
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1
- Culture medium different from test medium: Not stated
- Intervals of water quality measurement:0, 24, 48, 72 and 96 h
OTHER TEST CONDITIONS
- Adjustment of pH: Yes. pH 9.4 adjusted to pH 7.8 using concentrated hydrochloric acid
- Photoperiod: 16-hour light to 8 hour darkness photoperiod, with a 30 minute transition period.
- Light intensity: 50 to 500 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, Symptoms of intoxication: strongly extended gills, apathy, distended abdomen, fish mainly at bottom of the aquarium, exophthalmus, convultions, fish mainly at the water surface, mucus secretion, fish lying on side or back on the bottom, tumbling during swimming, changed body colour; observed at 2, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Limit test
- Justification for using less concentrations than requested by guideline: Based on the results of the range finding test, no mortalities or sub-lethal effects were obseved at maximum concentration, from the OECD/EC Test Guidelines, of 100mg/L.
- Range finding study: Yes
- Test concentrations: Not stated
- Results used to determine the conditions for the definitive study: No mortalities observed up to 100 mg/l at the end of the study - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: None
- Observations on body length and weight: Not stated
- Other biological observations: No other effects
- Mortality of control: No mortality
- Other adverse effects control: None stated
- Abnormal responses: None stated
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None stated
- Effect concentrations exceeding solubility of substance in test medium: No - Sublethal observations / clinical signs:
Table 1: Mortality and observed symptoms of intoxication
Nominal Test Item Concentration (mg/L) Number of Affected fish*/number of dead fish Observation Time 2 hours 24 hours 48 hours 72 hours 96 hours Control 0/0 0/0 0/0 0/0 0/0 100 0/0 0/0 0/0 0/0 0/0 LC50 > 100 mg/L * number of dead fish plus number with intoxification symptoms
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality in controls and maintenance of steady test conditions (including test substance concentration >80% of nominal) were all fulfilled.
- Conclusions:
- A 96-h LC50 >100 mg/l in Danio rerio was obtained in a guideline test and in accordance with GLP. The result is taken to be reliable.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 8 Feb 2012 - 20 Nov 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- - Method: A primary stock solution was prepared by solving the test substance in THF. Secondary stock solutions were prepared by diluting the primary stock solution with THF. For preparation of the required test concentrations the secondary stock solutions were diluted with well water. The test solutions were stirred overnight to allow a complete hydrolysis.
- Chemical name of vehicle: tetrahydrofuran (THF)
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.10 mL/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no visible undissolved test substance following preparation - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Strain: SMV Lot No. 12A121
- Source: TroutLodge, Inc., Sumner, Washington
- Age at study initiation (mean and range, SD):
- Length at study initiation (mean, range): 39 mm (35 - 42 mm)
- Weight at study initiation (mean and range): 0.69 g (0.50 - 0.87 g)
- Feeding during test: fish were inadvertently fed during the 24 hours prior to test initiation
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): same
- Type and amount of food: Trout Chow, ad libitum
- Feeding frequency: daily
- Health during acclimation: no mortalitiy observed 48 h prior to testing - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 64 mg/L (CaCO3)
- Test temperature:
- 14 - 15 °C
- pH:
- 6.9 - 7.5
- Dissolved oxygen:
- 5.4 - 9.6 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentrations:
3.1, 6.3, 13, 25 and 50 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Type: open
- Material, size, fill volume: glass, 39 × 20 × 25 centimeters, 10 L fill volume
- Aeration:
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.46 g /L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory well water
- Total organic carbon: 0.10 - 0.66 mg/L
- Alkalinity: as CaCO3: 20 mg/L
- Conductivity: 330 μS/cm
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Adjustment of pH: 25 and 50 mg/L solutions were adjusted from a pH of 8.4 to 7.5 and from 9.2 to 7.25, respectively, with 0.20 and 0.64 mL of 5.0 N hydrochloric acid
- Photoperiod: 16:8 h day-night regime
- Light intensity: 810 - 880 lux
EFFECT PARAMETERS MEASURED:
Mortaility and symptoms of intoxication after 0, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.0010, 0.010, 0.10, 1.0 and 10 mg/L, a control and a solvent (THF) control
- Results used to determine the conditions for the definitive study: no mortaility observed in any of the test concentrations - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- parent material
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- parent material
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- parent material
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: none
- Mortality of control: no
The average measured TOC concentrations in solvent control and treatment solutions ranged from 20 to 30 mg C/L. A concentration gradient was not observed. The comparable TOC content in all treatments and the solvent control indicates that the measured organic carbon originated from the solubiliser THF. The concentration of the hydrolysis products of the test substance could not be determined. - Reported statistics and error estimates:
- The LC50 was calculated using the computer program CETISTM (Ives, 2011)
Referenceopen allclose all
Description of key information
LC50 (96 h) > 100 mg/L (OECD 203, B. rerio, read across from CAS No. 227085-51-0), equivalent to > 81 mg/L when expressed in terms of the silanol hydrolysis product of the source substance, N-ethyl-3-trihydroxysilyl-2-methylpropanamine
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 81 mg/L
Additional information
A 96 hour LC50 value of > 50 mg/l and NOEC of ≥ 50 mg/l have been determined for the effects of the test substance on mortality of Oncorhynchus mykiss (OECD 203). The corresponding mean measured concentration of the substance in the treated test medium over the course of the test could not be determinated. No effects could be detected up to the highest tested concentration but the maximum nominal concentration used (50 mg/L) was well below the predicted solubility of the substance (13000 mg/L) and so does not provide an accurate representation of the likely toxicity of the substance.
Therefore it was considered appropriate to read across from the structurally analogue substance N-ethyl-3-trimethoxysilyl-2-methylpropanamine (CAS No. 227085-51-0). A limit test was conducted at a test concentration of 100 mg/L (OECD 203).
No effects were observed at this concentration to Zebra Fish (Brachydanio rerio). As the substance is subject to rapid hydrolysis, it is therefore likely that the test organisms were also exposed to hydrolysis products retained in the test media.
The results may be expressed in terms of concentration of the silanol hydrolysis product, N-ethyl-3-trihydroxysilyl-2-methylpropanamine, by applying a molecular weight correction: (MW of silanol (source) = 179.29 / MW of parent (source) = 221.37) * 100 = 81 mg/L.
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