Butan-2-one O,O',O''-(vinylsilylidyne)trioxime

Hydrolysis

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

February 10, 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

According to EU Method C.7, with GLP.

Qualifier:

according to guideline

Guideline:

EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)

Deviations:

no

GLP compliance:

yes

Radiolabelling:

no

Analytical monitoring:

yes

Details on sampling:

- Sampling intervals for the parent/transformation products: the concentrations of the 3 main components of the test substance were determined at the earliest possible time, i.e. 0.75 h after the start of the dissolution. Later sampling times were not required as the concentrations were already below the limit of quantification at 0.75 h.

Buffers:

Buffer pH 4:
- pH (actual pH preliminary test): 4.0
- Composition of buffer: 250 mL 0.1 mol/L potassium dihydrogencitrate (Fluka; Lot. No. 364546/1) and 45 mL 0.1 mol/L sodium hydroxide (Titrisol; Merck, Lot. No. OC489094) filled up to 500 mL with water.

Buffer pH 7:
- pH (actual pH preliminary test): 7.0
- Composition of buffer: 250 mL 0.1 mol/L potassium dihydrogenphosphate (Merck, Lot. No. A531473427) and 148.2 mL 0.1 mol/L sodium hydroxide filled up to 500 mL with water.

Buffer pH 9:
- pH (actual pH preliminary test): 9.0
- Composition of buffer: 250 mL 0.1 mol/l boric acid (Merck, Lot. No. K20380665) in 0.1 mol/L potassium chloride (Merck, Lot. No. K31517236313) and 106.5 mL 0.1 mol/L sodium hydroxide filled up to 500 mL with water.

The water (for analysis) Merck Lot. No. OC409681 was autoclaved before use.

Duration:

0.75 h

pH:

4.02

Temp.:

25 °C

Initial conc. measured:

9.41 mg/L

Duration:

0.75 h

pH:

7.01

Temp.:

25 °C

Initial conc. measured:

10.34 mg/L

Duration:

0.75 h

pH:

8.98

Temp.:

25 °C

Initial conc. measured:

9.78 mg/L

Number of replicates:

There were no replicates

Positive controls:

no

Negative controls:

no

Preliminary study:

The preliminary test indicated that the half-lives of the 3 main components of the test substance at 25 °C and at pH 4, pH 7 and pH 9 are shorter than 1 hour in each case. Therefore the test substance is considered to be easily hydrolysable and no main test was performed, according to the guideline.

Test performance:

The concentrations of the 3 main components of the reaction mass were determined at the earliest possible time, i.e. 0.75 h after the start of the dissolution. Later sampling times were not required as the concentrations were already below the limit of quantification at 0.75 h.

Transformation products:

no

Details on hydrolysis and appearance of transformation product(s):

The test substance is composed of several compounds, including the 3 main components acetone O,O'-[methoxy(vinyl)silanediyl]oxime; acetone O,O',O''-(vinylsilanetriyl)oxime and acetone O-[dimethoxy(vinyl)silyl]oxime . They polymerise, triggered by hydrolysis.

Key result

pH:

4

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: acetoneO,O',O''-(vinylsilanetriyl)oxime

Key result

pH:

7

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: acetoneO,O',O''-(vinylsilanetriyl)oxime

Key result

pH:

9

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: acetoneO,O',O''-(vinylsilanetriyl)oxime

Other kinetic parameters:

No kinetic of the hydrolysis could be determined because of the slow dissolution of the test substance and the fast hydrolysis.

Rate constants of hydrolysis and half life times

pH	temperature (°C)	rate constant (h^-1)	rate constant (s^-1)	half life (h)
4	25	>1	>0.0003	<1
7	25	>1	>0.0003	<1
9	25	>1	>0.0003	<1

Results of the hydrolysis at 25 °C, pH = 4 (preliminary test)

time of exposure [h]	pH	concentration [mg/L]	decrease of the initial concentr. [%]
0	4.02	9.41*	/
0.75	4.02	< 3.78 **	>55

Results of the hydrolysis at 25 °C, pH = 7 (preliminary test)

time of exposure [h]	pH	concentration [mg/L]	decrease of the initial concentr. [%]
0	7.01	10.34 *	/
0.75	7.01	< 3.78 **	>55

Results of the hydrolysis at 25 °C, pH = 9 (preliminary test)

time of exposure [h]	pH	concentration [mg/L]	decrease of the initial concentr. [%]
0	8.98	9.78 *	/
0.75	8.98	< 3.78 **	>55

* Concentration calculated from the nominal initial concentration of the test substance and the content of the component.

** Below the limit of quantification.

Validity criteria fulfilled:

yes

Conclusions:

The analysed concentration of acetone O,O',O''-(vinylsilanetriyl)oxime was below the limit of quantification (0.94 mg/mL) at 0.75 h after starting the dissolution of the test substance. More than 50 % of this component at pH 4, pH 7 and pH 9 hydrolysed in less than 1 hour at 25 °C.

Executive summary:

The hydrolysis as a function of pH study was carried out for the test substance. This test material is composed of several compounds, including the 3 main components acetone O,O'-[methoxy(vinyl)silanediyl]oxime; acetone O,O',O''-(vinylsilanetriyl)oxime and acetoneO-[dimethoxy(vinyl)silyl]oxime. They polymerise, triggered by hydrolysis. The rate constants of hydrolysis and half-life times of the 3 main components were determined at pH 4, pH 7 and pH 9. Due to the predicted instability of the test substance, the test was performed at 25 °C and not at 50 °C as described in the guideline for the "preliminary test". For the acetone O,O',O''-(vinylsilanetriyl)oxime a component of the test substance, no "main test" was performed as the half-life time was < 1 h. The analysed concentration by gas chromatography, was below the limit of quantification (0.94 mg/mL) at 0.75 h after starting the dissolution of the test substance. More than 50 % of this component at pH 4, pH 7 and pH 9 hydrolysed in less than 1 hour at 25 °C. Acetone O,O',O''-(vinylsilanetriyl)oxime, one of the main components of the test substance is easily hydrolysable at each of the pH's tested.

Reference 2

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

February 9, 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

According to EU Method C.7, with GLP.

Qualifier:

according to guideline

Guideline:

EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)

Deviations:

no

GLP compliance:

yes

Radiolabelling:

no

Analytical monitoring:

yes

Details on sampling:

- Sampling intervals for the parent/transformation products: the concentrations of the 3 main components MMAC2, MAC3 and MM2AC of the test substance were determined at the earliest possible time, i.e. 0.75 h after the start of the dissolution. Later sampling times were not required as the concentrations were already below the limit of quantification at 0.75 h.

Buffers:

Buffer pH 4:
- pH (actual pH preliminary test): 4.0
- Composition of buffer: 250 ml 0.1 mol/l potassium dihydrogencitrate (Fluka; lot. No. 364546/1) and 45 mL 0.1 mol/l sodium hydroxide (Titrisol; Merck, Lot. No. OC489094) filled up to 500 ml with water.

Buffer pH 7:
- pH (actual pH preliminary test): 7.0
- Composition of buffer: 250 ml 0.1 mol/l potassium dihydrogenphosphate (Merck, lot. No. A531473427) and 148.2 ml 0.1 mol/l sodium hydroxide filled up to 500 ml with water.

Buffer pH 9:
- pH (actual pH preliminary test): 9.0
- Composition of buffer: 250 ml 0.1 mol/l boric acid (Merck, lot. No. K20380665) in 0.1 mol/l potassium chloride (Merck, lot. No. K31517236313) and 106.5 ml 0.1 mol/l sodium hydroxide filled up to 500 ml with water.

The water (for analysis; Merck; lot. No. OC409681 was autoclaved before use.

Details on test conditions:

Preparation of the test medium:

The hydrolysis assay should be performed with a concentration of the test substance of ca. 50 % saturation. The water solubility of the test substance could not be determined, because of the rapid hydrolysis. A concentration of nominally 50 mg test substance per litre was selected, as this concentration is within the range of the calculated results and as a clear solution (checked by eye) was obtained.

Solutions of the test substance were prepared by dissolving 24.9, 24.1 and 24.5 mg respectively of the test substance in 100 mL of the appropriate buffers and 400 mL water (for analysis, Merck, Lot No. OC522462), using ultrasonication. Afterwards nitrogen was bubbled through each solution to expel oxygen. The solutions were kept for fifteen minutes in a water bath (DL 30 V26 1, accuracy ± 0.01 °C; Haake, Karlsruhe, Germany) at 25.0 °C in the dark.

Duration:

0.75 h

pH:

4.01

Temp.:

25 °C

Initial conc. measured:

11.95 mg/L

Duration:

0.75 h

pH:

6.99

Temp.:

25 °C

Initial conc. measured:

11.57 mg/L

Duration:

0.75 h

pH:

8.98

Temp.:

25 °C

Initial conc. measured:

11.81 mg/L

Number of replicates:

There were no replicates.

Positive controls:

no

Negative controls:

no

Preliminary study:

The preliminary test indicated that the half-lifes of the 3 main components MMAC2, MAC3 and MM2AC of the test substance at 25 °C and at pH 4, pH 7 and pH 9 were shorter than 1 hour in each case. Therefore the test substance is considered to be easily hydrolysable and no main test was performed, according to the guideline.

Test performance:

The concentrations of the 3 main components of the test substance were determined at the earliest possible time, i.e. 0.75 h after the start of the dissolution. Later sampling times were not required as the concentrations were already below the limit of quantification at 0.75 h.

Transformation products:

no

Details on hydrolysis and appearance of transformation product(s):

The test substance is composed of several compounds, including the 3 main components MMAC2, MAC3 and MM2AC. They polymerise, triggered by hydrolysis.

Key result

pH:

4

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Propan-2-one-O,O',O''-(methylsilantriyl)trioxime

Key result

pH:

7

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Propan-2-one-O,O',O''-(methylsilantriyl)trioxime

Key result

pH:

9

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Propan-2-one-O,O',O''-(methylsilantriyl)trioxime

Other kinetic parameters:

No kinetic of the hydrolysis could be determined because of the slow dissolution of the test substance and the fast hydrolysis.

Results of Wasox-MMAC2 component Propan-2-one-O,O',O''-(methylsilantriyl)trioxime (MAC3):

Rate constants of hydrolysis and half life times

pH	temperature (°C)	rate constant (h^-1)	rate constant (s^-1)	half life (h)
4	25	>1	>0.0003	<1
7	25	>1	>0.0003	<1
9	25	>1	>0.0003	<1

Results of the hydrolysis at 25 °C, pH = 4 (preliminary test)

time of exposure [h]	pH	concentration [mg/L]	decrease of the initial concentr. [%]
0	4.01	11.95 *	/
0.75	4.01	< 4.8 **	>55

Results of the hydrolysis at 25 °C, pH = 7 (preliminary test)

time of exposure [h]	pH	concentration [mg/L]	decrease of the initial concentr. [%]
0	6.99	11.57 *	/
0.75	6.99	< 4.8 **	>55

Results of the hydrolysis at 25 °C, pH = 9 (preliminary test)

time of exposure [h]	pH	concentration [mg/L]	decrease of the initial concentr. [%]
0	8.98	11.81 *	/
0.75	8.98	< 4.83 **	>55

* Concentration calculated from the nominal initial concentration of the test substance and the content of the component.

** Below the limit of quantification.

Validity criteria fulfilled:

yes

Conclusions:

The Propan-2-one-O,O',O''-(methylsilantriyl)trioxime concentration was below the limit of quantification (1.04 mg/mL) at 0.75 h after starting the dissolution of the test substance. More than 50 % of this component at pH 4, pH 7 and pH 9 hydrolysed in less than 1 hour at 25 °C.

Executive summary:

The hydrolysis as a function of pH study was carried out for the test substance Wasox-MMAC2 according to EU Method C.7. This test material is composed of several compounds, including the 3 main components MMAC2 (Propan-2-one-O,O'-(methoxymethylsilandiyl) dioxime), MAC3 (Propan-2-one-O,O',O''-(methylsilantriyl)trioxime) and MM2AC (Propan-2-one-O-(dimethoxymethylsilyl)oxime). They polymerise, triggered by hydrolysis.

The rate constants of the 3 main components were determined at pH 4, pH 7 and pH 9. Due to the predicted instability of the test substance, communicated by the sponsor, the test was performed at 25°C and not at 50°C as described in the guideline for the "preliminary test". No "main test" was performed in agreement with the guideline, as the half-life time was < 1 h in each case. The test substance dissolved slowly in water, and the hydrolysis was fast, therefore the concentration was determined only at one time (0.75 h) after the start of the dissolution. The chemical analysis was performed by gaschromatography. Each of the analysed concentrations was below the limit of quantification at 0.75 h after starting the dissolution of the test substance. More than 50 % of the component MAC3 (Propan-2-one-O,O',O''-(methylsilantriyl)trioxime) of the test substance at pH 4, pH 7 and pH 9 hydrolysed in less than 1 hour at 25°C. Therefore, the substance is easily hydrolysable at test conditions.

 

Reference 3

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

According to OECD Guideline 111. GLP study.

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Deviations:

yes

Remarks:

(test concentration 0.01 mol/L, see justification in section "Details on test conditions" )

Qualifier:

according to guideline

Guideline:

EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)

Deviations:

yes

Remarks:

(test concentration 0.01 mol/L, see justification in section "Details on test conditions" )

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

MAIN TEST (NMR Spectrometry)
- Sampling intervals for the parent/transformation products: Measurements were performed 150 seconds after the initial contact of the test item with the aqueous phases. Number of scans were set to 16 (total determination time of approx. 180 seconds).
- Sampling method: Sampling was optimized for shortest sample preparation times to be able to start the measurements as quickly after the first contact of the test item with water as possible. In general first the test item was weighted exactly (to the nearest of 0.1 mg) into dry NMR tubes. Then the needed volumes of the previously prepared buffer solutions in D2O were measured in disposable 1 mL syringes and added in one portion into the NMR tubes. The tubes were then closed with a plastic cap and mixed thoroughly. Afterwards the tubes were placed into the spinner and inserted into the NRM spectrometer by automation. The average time from the initial contact of the test item with water until the beginning of the measurements was determined to be 150 seconds.
STEP WISE HYDROLYSIS (in acetone-d6)
A series of sample of the test item in acetone-d6 were prepared and increasing amounts of the 10 mg/g D2O stock solution in acetone-d6 were added. This addition of defined amounts of water led to further gradual hydrolysis resulting in a further decrease in the unhydrolysed acetoxy signal and a further increase in the signal of free acetic acid.

Buffers:

- pH: 1.2, 4.0, 7.0 and 9.0
- Composition of buffer:
pH 1.2: 19.06 mg of potassium chloride and 10.0 mg of 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid sodium salt were dissolved in 5 mL of deuterium oxide. The pH of the buffer was adjusted to 1.2 with deuterium chloride.
pH 4.0: 68.53 mg of potassium phosphate monobasic and 10.3 mg of 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid sodium salt were dissolved in 5 mL of deuterium oxide. The pH of the buffer was adjusted to 4.0 with deuterium chloride.
pH 7.0: 136.71 mg of potassium phosphate monobasic and 20.4 mg of 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid sodium salt were dissolved in 10 mL of deuterium oxide. The pH of the buffer was adjusted to 7.0 with deuterium chloride.

Details on test conditions:

TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: NMR tubes and standard laboratory glassware for stock solution preparation.
- Sterilisation method: Due to the fast hydrolysis rates of the test item an biotic degradation does not have to be prevented by any special procedures. Anyhow only brand new NMR tubes were used and all other glassware and materials which were in contact with the buffering and measuring solutions were cleaned thoroughly in a laboratory cleaning machine at 80°C.
TEST MEDIUM
- Volume used/treatment: 1 mL.
- Preparation of test medium:
Main test (NMR spectrometry): Approximately 25 mg of test item was weighted (to the nearest of 0.1 mg) exactly into dry NMR tubes. Then the needed volumes of the previously prepared buffer solutions in D2O were measured to give concentrations of 0.1 mol/L in disposable 1 mL syringes.
Step wise hydrolysis (in acetone-d6): A series of sample weights containing 24.4 g to 25.5 g of the test item in acetone-d6 were prepared and increasing amounts of the 10 mg/g D2O stock solution in acetone-d6 were added. The amount of pure acetone-d6 for dissolving the test item was chosen individually for each sample to give a total volume of 1 mL (including the acetone-d6 from the D2O stock solutions). The added amounts of water were in the range of 20.2 mg to 895.5 mg of the D2O stock solution per NMR tube, corresponding to 0.226 mg to 10.012 mg of pure water per tube.
- Identity and concentration of co-solvent: Deuterium oxide and acetone-d6.
TEST CONCENTRATION (MAIN TEST): 0.1 mol/L.
According to the test guideline the maximum concentration of the test item should not exceed 0.01 mol/L (= 2.48 g/L for the test item). NMR spectroscopy is an analytical method that is optimized for the determination of chemical structures, not for trace analysis of substances. Therefore the sensitivity of NMR spectroscopy is much lower than the sensitivity of e. g. HPLC-UV methods. Good signal intensities can be reached with concentrations above 0.01 mol/L as the minimum concentrations. Due to the expected fast hydrolysis rates concentrations of the test item should be chosen high enough to be able to quantify at least remaining 10% of the initial concentration. Therefore to be able to reach an acceptable sensitivity of the NMR method the sample weights have to be increased compared to the values given in the OECD guideline 111. Approximately 25 mg of the test item have to be weighed into 1 mL of the NMR solvents to give concentrations of 0.1 mol/L which is 10 fold the concentration given in the guideline. A reduction of concentrations is technically not possible.

Duration:

3 min

Initial conc. measured:

0.1 mol/L

Number of replicates:

1 replicate.

Positive controls:

no

Negative controls:

no

Preliminary study:

The preliminary test was not performed due to the known instability of the test item.

Transformation products:

yes

No.:

#1

No.:

#2

Details on hydrolysis and appearance of transformation product(s):

Step wise hydrolysis of the test item in acetone-d6:
The mechanism of hydrolysis was analyzed by visualizing the signal intensities of the unhydrolysed acetoxy signal and the signal of free acetic acid versus the molar amount of additional water. The areas of these two signals correlated in a reciprocal way. The reduction of unhydrolysed test item caused an increase in free acetic acid concentration. By this experiment it was proved, that the test item reacts to form free acetic acid as soon as it gets in contact with water. It could also be seen that the increase in free acetic acid concentration was not linear compared to the added amount of water. What could be observed at low amounts of water added was an increase in the free acetic acid concentration that even was higher than the molar amount of water added. Hence one molecule of water seems to be able to hydrolyse more than one acetoxy group of the test item. This observation is only explainable by arguing with the formation of dimers or even oligomers of the hydrolysis products (silanols). A hydrolysed free silanol (R3Si-OH) contains an available free alcohol functionality which itself can lead to hydrolysis of another unhydrolysed acetoxy group. With increasing amounts of hydrolysed acetoxy groups the probability of further intermolecular hydrolysis reactions decreases. This experiment proved the stated mechanism of hydrolysis – with free acetic acid as the main hydrolysis product – and it also showed the presence as well as the fast kinetics of polycondensation reactions of the forming silanols.

Key result

pH:

1.2

Temp.:

22 °C

DT50:

< 0.625 min

Key result

pH:

4

Temp.:

22 °C

DT50:

< 0.625 min

Key result

pH:

7

Temp.:

22 °C

DT50:

< 0.625 min

Key result

pH:

9

Temp.:

22 °C

DT50:

< 0.625 min

Other kinetic parameters:

Due to the extremely fast hydrolysis reaction of propyltriacetoxysilane, a detailed determination of rate constants and half life times is technically not possible.

Details on results:

MAIN TEST (NMR spectrometry):
TEST CONDITIONS
- pH: 1.2, 4, 7, 9
- Temperature: 22 ± 2 ºC

MAJOR TRANSFORMATION PRODUCTS
Independent from the pH of the buffering solutions the samples showed clear spectra with 100% of free acetic acid (integrals of 8.9679 to 9.0092) and none of the unhydrolysed test item remaining. In analogy to the tests in acetone-d6 (see section "details on hydrolysis and appearance of transformation products"), the unhydrolysed test item must have given a signal with a different chemical shift than the signal of free acetic acid. Due to the fast hydrolysis this signal could never be observed. Therefore hydrolysis was finished – independent from pH – at least 150 seconds after the initial contact of the test item with water. Assuming that a minimum of four half life cycles were completed during the time from the initial contact of the test item with water until the beginning of the measurement, the maximum half life time of the test item would be 150 sec / 4 = 37.5 sec.

PATHWAYS OF HYDROLYSIS
- Description of pathways: The step wise hydrolysis of the test item in acetone-d6 proved the stated mechanism of hydrolysis – with free acetic acid as the main hydrolysis product – and it also showed the presence as well as the fast kinetics of polycondensation reactions of the forming silanols.
- Figures of chemical structures attached: No

Main test (NMR spectrometry):

The final results of the determination of hydrolysis rates are given in the following table:

 

pH value	Estimated half-life (mean 22°C)
1.2	< 37.5 seconds
4.0	< 37.5 seconds
7.0	< 37.5 seconds
9.0	< 37.5 seconds

Step wise hydrolysis of the test item in acetone-d6:

Signal areas of the test item and acetic acid in the step wise hydrolysis:

Peak Area Test Item	Peak Area Free Acetic acid	Mol% of water
6.5391	1.8949	0
5.6321	2.4678	2.86
5.1779	2.8543	5.9
4.5423	3.7352	9.32
4.1191	4.3717	12.67
2.8151	5.7787	25.71
1.8272	7.0574	40.94
1.2529	8.027	59.15
0	8.9155	124.11

Validity criteria fulfilled:

yes

Conclusions:

The half-life of test item propyltriacetoxysilane was determined to be < 37.5 seconds at pH 1.2, 4, 7 and 9. The stated mechanism of hydrolysis of propyltriacetoxysilane was free acetic acid as the main hydrolysis product and the fast kinetic polycondensation reaction of the forming silanols (trihydroxypropylsilane).

Executive summary:

The abiotic degradation of the test item propyltriacetoxysilane was determined according to OECD-Guideline 111 and in accordance with European Commission Council Regulation (EC) No 440/2008, Annex, Part C, method C.7. Due to the fast kinetics of the hydrolysis of the test item, half-life times and hydrolysis rate constants could not be determined properly with common analytical methods.

While the kinetics of the hydrolysis reaction was too fast to be observed, the mechanism of the reaction could be proved by a stepwise NMR experiment. For this experiment, increasing amounts of water (D2O) were added to solutions of the test item in a dry organic solvent (acetone-d6). The stepwise hydrolysis with formation of acetic acid as the main hydrolysis product could be shown by this set up. It could also be shown the presence as well as the fast kinetics of polycondensation reactions of the forming silanols. In addition to this, the degradation in buffered aqueous solutions at different pH values (1.2, 4, 7 and 9) was investigated. All tests were performed at a temperature of 22°C (± 2°C) using 1H-NMR experiments. Measurements were performed 150 seconds after the initial contact of the test item with the aqueous phases. Independent from pH, after a reaction time of 150 seconds, none of the initially present test item could be measured in the aqueous solutions, thus total hydrolysis is completed at least 150 seconds after the initial contact of the test item with water. With respect to the detection limits of the 1H-NMR method, a minimum of four completed half-life cycles can be estimated for the test item in a reaction time of 150 seconds. Therefore a maximum half life time of <37.5 seconds can be estimated for test item propyltriacetoxysilane. The half life times of the test item – with respect to the minimum time scale of the analysis – are independent from pH.

Reference 4

Endpoint:

hydrolysis

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH
The analogue VAC3 component of the reaction mass Wasox-VMAC2, has a molecular structure similar to butan-2-one O,O',O''-(vinylsilanetriyl)oxime and therefore, they also have comparable values for the relevant molecular properties for abiotic degradation.
See attached reporting format.

Reason / purpose for cross-reference:

read-across source

Preliminary study:

Based on read-across approach from analogue substance VAC3 (component of reaction mass Wasox-VMAC2), the preliminary test indicated that the half-life was shorter than 1 hour at 25 °C and at pH 4, pH 7 and pH 9. Therefore, it was considered to be easily hydrolysable and no main test was performed, according to the guideline.

Test performance:

Based on read-across approach from analogue substance VAC3 (component of reaction mass Wasox-VMAC2), the concentration was determined at the earliest possible time, i.e. 0.75 h after the start of the dissolution. Later sampling times was not required as the concentrations was already below the limit of quantification at 0.75 h.

Transformation products:

no

Key result

pH:

4

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: (results based on analogue substance acetone O,O',O''-(vinylsilanetriyl)oxime)

Key result

pH:

7

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: (results based on analogue substance acetone O,O',O''-(vinylsilanetriyl)oxime)

Key result

pH:

9

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: (results based on analogue substance acetone O,O',O''-(vinylsilanetriyl)oxime)

Other kinetic parameters:

Based on read-across approach from analogue substance, no kinetic of the hydrolysis could be determined because of the slow dissolution of the test substance and the fast hydrolysis.

Details on results:

Based on read-across from experimental results with analogue substance VAC3 (component of reaction mass Wasox-VMAC2), the test item butan-2-one O,O',O''-(vinylsilanetriyl)oxime is determined to hydrolyse in less than 1 hour at 25 ºC.

Validity criteria fulfilled:

not applicable

Conclusions:

Based on the read-across approach from experimental results obtained with analogue VAC3, butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically instable with a low half-life.

Executive summary:

Based on the experimental results obtained with analogue substance VAC3 (half-life <1 hour at pH 4, 7 and 9 at 25 ºC), the read-across approach was applied and butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically instable with a low half-life.

Reference 5

Endpoint:

hydrolysis

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH
The analogue MAC3 component of the reaction mass Wasox-MMAC2, has a molecular structure similar to butan-2-one O,O',O''-(vinylsilanetriyl)oxime and therefore, they also have comparable values for the relevant molecular properties for abiotic degradation.
See attached reporting format.

Reason / purpose for cross-reference:

read-across source

Preliminary study:

Based on read-across approach from analogue substance MAC3 (component of reaction mass Wasox-MMAC2), the preliminary test indicated that the half-life was shorter than 1 hour at 25 °C and at pH 4, pH 7 and pH 9. Therefore, it was considered to be easily hydrolysable and no main test was performed, according to the guideline.

Test performance:

Based on read-across approach from analogue substance MAC3 (component of reaction mass Wasox-MMAC2), the concentration was determined at the earliest possible time, i.e. 0.75 h after the start of the dissolution. Later sampling times was not required as the concentrations was already below the limit of quantification at 0.75 h.

Transformation products:

no

Key result

pH:

4

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: (results based on analogue substance Propan-2-one-O,O',O''-(methylsilantriyl)trioxime)

Key result

pH:

7

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: (results based on analogue substance Propan-2-one-O,O',O''-(methylsilantriyl)trioxime)

Key result

pH:

9

Temp.:

25 °C

Hydrolysis rate constant:

> 1 h-1

DT50:

< 1 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: (results based on analogue substance Propan-2-one-O,O',O''-(methylsilantriyl)trioxime)

Other kinetic parameters:

Based on read-across approach from analogue substance, no kinetic of the hydrolysis could be determined because of the slow dissolution of the test substance and the fast hydrolysis.

Details on results:

Based on read-across from experimental results with analogue substance MAC3 (component of reaction mass Wasox-MMAC2), the test item butan-2-one O,O',O''-(vinylsilanetriyl)oxime is determined to hydrolyse in less than 1 hour at 25 ºC.

Validity criteria fulfilled:

not applicable

Conclusions:

Based on the read-across approach from experimental results obtained with analogue MAC3, butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically instable with a low half-life.

Executive summary:

Based on the experimental results obtained with analogue substance MAC3 (half-life <1 hour at pH 4, 7 and 9 at 25 ºC), the read-across approach was applied and butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically instable with a low half-life.

Reference 6

Endpoint:

hydrolysis

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH
The analogue Propyltriacetoxysilane has a molecular structure similar to butan-2-one O,O',O''-(vinylsilanetriyl)oxime and therefore, they also have comparable values for the relevant molecular properties for abiotic degradation.
See attached reporting format.

Reason / purpose for cross-reference:

read-across source

Preliminary study:

The preliminary test on analogue propyltriacetoxysilane was not performed due to the known instability of the test item.

Transformation products:

yes

No.:

#1

No.:

#2

Details on hydrolysis and appearance of transformation product(s):

Based on the experimental results with analogue propyltriacetoxysilane and applying the read-across approach, it was determined that butan-2-one O,O',O''-(vinylsilanetriyl)oxime hidrolyses into free butanone oxime as the main hydrolysis product and silanols with fast kinetics of polycondensation reactions.

Key result

pH:

1.2

Temp.:

22 °C

DT50:

< 0.625 min

Remarks on result:

other: (results obtained with analogue substance propyltriacetoxysilane)

Key result

pH:

4

Temp.:

22 °C

DT50:

< 0.625 min

Remarks on result:

other: (results obtained with analogue substance propyltriacetoxysilane)

Key result

pH:

7

Temp.:

22 °C

DT50:

< 0.625 min

Remarks on result:

other: (results obtained with analogue substance propyltriacetoxysilane)

Key result

pH:

9

Temp.:

22 °C

DT50:

< 0.625 min

Remarks on result:

other: (results obtained with analogue substance propyltriacetoxysilane)

Other kinetic parameters:

Due to the extremely fast hydrolysis reaction of analogue propyltriacetoxysilane, a detailed determination of rate constants and half-life times was technically not possible.

Details on results:

Based on experimental results obtained with analogue propyltriacetoxysilane where hydrolysis was finished at least 150 seconds after the initial contact of the test item with water and the maximum half life time of test item was determined to be 37.5 seconds at pH 1.2, 4, 7 and 9, the read-across approach was applied and butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically unstable, with a fast hydrolysis and low half-life regardless of pH.

Validity criteria fulfilled:

not applicable

Conclusions:

Based on the read-across approach from experimental results on analogue propyltriacetoxysilane, butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically unstable, with a fast hydrolysis (very low half-life time) into butanone oxime and silanols regardless of pH.

Executive summary:

The abiotic degradation of analogue substance propyltriacetoxysilane was performed according to OECD Guideline 111. The stepwise hydrolysis with formation of acetic acid as the main hydrolysis product and the presence as well as the fast kinetics of polycondensation reactions of the forming silanols (trihydroxypropylsilane) could be observed. The hydrolysis of analogue propyltriacetoxysilane was completed at least 150 seconds at pH 1.2, 4, 7 and 9 after the initial contact of the test item with water. A minimum of four completed half life cycles were estimated for the test item in a reaction time of 150 seconds and therefore the maximum half life time of <37.5 seconds was estimated for propyltriacetoxysilane. Based on these results, the read-across approach was applied and butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be rapidly hydrolysed into butanone oxime and corresponding silanols in contact with water regardless of pH.

Description of key information

Weight of evidence: Read-across approach from analogue VAC3 (OECD 111 and GLP): Butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically instable with a very low half-life.

Weight of evidence: Read-across approach from analogue MAC3 (OECD 111 and GLP): Butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically instable with a very low half-life.

Weight of evidence: Read-across approach from analogue propyltriacetoxysilane (OECD 111 and GLP): Butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically unstable, with a fast hydrolysis (very low half-life time) into butanone oxime and silanols regardless of pH.

Additional information

Read-across approach from experimental results on analogue substance VAC3:

Weight of evidence: Based on the experimental results obtained with analogue substance VAC3 in a study performed according to OECD 111 and GLP (half-life <1 hour at pH 4, 7 and 9 at 25 ºC), the read-across approach was applied and butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically instable with a low half-life.

Read-across approach from experimental results on analogue substance MAC3:

Weight of evidence: Based on the experimental results obtained with analogue substance MAC3 in a study performed according to OECD 111 and GLP (half-life <1 hour at pH 4, 7 and 9 at 25 ºC), the read-across approach was applied and butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically instable with a low half-life.

Read-across approach from experimental results on analogue substance propyltriacetoxysilane:

Weight of evidence: Based on the read-across approach from experimental results obtained in a study performed in accordance with OECD 111 and GLP on analogue propyltriacetoxysilane (half-life <37.5 seconds at 25 ºC regardless pH), butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be hydrolytically unstable, with a fast hydrolysis (very low half-life time) into butanone oxime and silanols.