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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 (2010) was carried out with the test substance trans-N1,N1,N4,N4-tetrakis(2-hydroxyethyl)-1,4-cyclohexanedicarboxamide. Test system was activated sludge of the municipal treatment plant of D-31137 Hildesheim. The test was carried out under static conditions with the limit concentration 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The inhibition of respiration for the test item replicates ranged from -4 to -6 %. The test substance caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit concentration of 1000 mg/L. Thus the NOEC is 1000 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L
EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information