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EC number: 256-360-6
CAS number: 48145-04-6
Prior to use, the master strains were checked for characteristics,
viability and spontaneous reversion rate (all were found to be
satisfactory). The amino acid supplemented top agar and S9‑mix used in
both experiments was shown to be sterile. The culture density for each
bacterial strain was also checked and considered acceptable.
A history profile of vehicle, untreated and positive control
values for 2010 and 2011 was included in the test report. The results of
this study was in accordance with the history profile.
2-phenoxyethyl acrylate was tested in the reverse mutation assay (Ames
Test) using Salmonella Typhimurium strains (TA1535, TA1537, TA98,
TA100) and Escherichia Coli strain (WP2uvrA). The test was performed in
accordance with OECD test guideline 471 and EU B13/14 using the plate
incorporation and pre-incubation methods at five dose levels, in
triplicate, both with and without the addition of rat liver homogenate
metabolosing system (10% liver S9 in standard co-factors). The dose
range was determined in a preliminary toxicity assay and was 50 to 5000
ug/plate in the first experiment.The experiment was repeated on a
seperate day (pre-incubatrion method) using the same dose range, fresh
cultures of the bacterial strains and fresh 2 -phenoxyethyl acrylate
No toxicological significant increases in the frequecy of revertant
colonies were recorded for any of the bacterial strains, with any dose
of 2-phenoxyethyl acrylate, either with or without metabolic activation
per exposure method, hence 2-phenoxyethyl acrylate was found to be
The three experimental studies available are of high reliability, GLP
studies with no or insignificant deviations.
All studies concluded that 2 -phenoxyethyl acrylate is non-genotoxic:
acrylate was found to be non-mutagenic in the reverse mutation assay
(Ames test) using Salmonella Typhimurium and Escherichia Coli. The study
was performed in accordance to OECD 471 using the plate incorporation
and pre-incubation methods at five dose levels (50 to 5000 ug/plate), in
triplicate, both with and without metabolic activation (10% liver S9 in
acrylate was non-mutagenic and did not induce any clastogenic effects to
L5178Y mouse lymphoma cells treated in vitro. The study was performed in
accordance to OECD 476. Two experiments were performed using dose levels
of 1.25 to 30 μg/ml in the absence of metabolic activation and 7.5 to
240 μg/ml in the presence of metabolic activation for Experiment 1
(4-hour exposure). In Experiment 2, the dose range was 0.63 to 40 μg/ml
in the absence of metabolic activation (24-hour exposure), and 60 to 240
μg/ml in the presence of metabolic activation (4-hour exposure).
acrylate was considered to be non-clastogenic and did not induce any
toxicological significant increases in the frequency of cells with
aberrations, when tested for the potential of inducing chromosome
aberrations in cultured mammalian cells (human lymphocytes) in vitro.
The study was performed in accordance to OECD 473. The study was
performed in accordance to OECD 473. Duplicate cultures of human
lymphocytes, treated with 2-phenoxyethyl acrylate were evaluated for
chromosome aberrations at three dose levels, together with vehicle and
positive controls. Three treatment conditions were used for the study,
i.e. In Experiment 1, 4 hours in the presence of an induced rat liver
homogenate metabolising system (S9), at a 2% final concentration with
cell harvest after a 20-hour expression period and a 4 hours exposure in
the absence of metabolic activation (S9) with a 20-hour expression
period. In Experiment 2, the 4 hours exposure with addition of S9 was
repeated (using a 1% final S9 concentration); whilst in the absence of
metabolic activation the exposure time was increased to 24 hours.
The available in vitro data suggest that 2-phenoxyethyl acrylate should
not be classified as mutagenic or genotoxic.
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