Registration Dossier

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: scientifically acceptable study report

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report Date:
1982

Materials and methods

Principles of method if other than guideline:
according to BASF-internal standard
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name of the test substance used in the study report: 2-Phenoxyethylacrylat
Purity: ca. 70%
Remaining acrylic acid < 1%

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female
Details on test animals and environmental conditions:
Acclimatization period: at least one week
Animal identification using cage cards (group identification).
Five animals per cage (type: stainless steel wire mesh cages).
Housing conditions: room temperature 20 - 26°C, relative humidity 45 - 75%, day/night rhythm 12 h / 12 h.
The animals were offered a standardized animal laboratory diet as well as tap water ad libitum. Food depreviation 16 hours before test substance application (water was available ad libitum).

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
olive oil
Details on exposure:
The test substance was insoluble in water.
Form of application: emulsion
Concentrations used: 4.3, 9.28 and 14% (w/v)
Application volume: 5 ml/kg
Doses:
215, 464 and 700 mg/kg
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 464 - < 700 mg/kg bw
Mortality:
215 and 464 mg/kg: no deaths; 700 mg/kg: 10/10 after 14 days
Clinical signs:
Dyspnea, apathy, abnormal position, staggering, atony, paresis of the hindlimbs, missing cornea and pain reflexes, narcotic-like state, trembling, spastic gait, stretching, ruffled fur, erythema, exsiccosis, paresis, ptosis, cachexia, poor general state
Body weight:
Mean body weight male animals: 28.0 g at study start, 36.9 g after 13 days
Mean body weight female animals: 25.3 g at study start, 28.9 g after 13 days
Gross pathology:
Animals that died: multiple intra-abdominal test substance residues
Sacrificed animals: no intra-abdominal test substance residues, no adherences

Applicant's summary and conclusion

Executive summary:

464 mg/kg < LD50 by i.p. in mice < 700 mg/kg