8,9,10-trinorborn-2-ene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: acceptable publication which meets basic scientic principles; basic data on expermimental design are given.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VII

Author:

Smyth, HF; Carpenter, CP; Weil, CS; Pozzani UC; Striegel JA; Nycum, JS

Year:

1969

Bibliographic source:

Amer. Ind. Hyg. Assoc. J. 30 (1969), 470

Report date:

1969

Reference 2

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VI

Author:

Smyth, HF; Carpenter, CP; Weil, CS; Pozzani UC; Striegel JA

Year:

1962

Bibliographic source:

Amer. Ind. Hyg. Assoc. J. 23 (1962), 95

Report date:

1962

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

non-fasted animals, limited reporting

GLP compliance:

no

Remarks:

pre-GLP study

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

8,9,10-trinorborn-2-ene

EC Number:

207-866-0

EC Name:

8,9,10-trinorborn-2-ene

Cas Number:

498-66-8

Molecular formula:

C7H10

IUPAC Name:

Bicyclo-[2.2.1]-hept-2-ene

Details on test material:

- name of test material as cited in study report: 2-Norbornene
- no further information on test substance

Test animals

Species:

rat

Strain:

other: Carworth-Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

Test Animals
Source: no data
Weight at initiation of study: 90-120 g
Age: 4-5 weeks
No fasting before commencement of study
housing: no data
acclimatation: no data
Diet and Water ad libidum

environmental conditions:
no data on humidity, temperature, airchange or lightning conditions.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Doses:

At least 4 dose levels in a logarithmic scale differing by a factor of 2. Individual doses not specified

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

observation period following administration:14d (no data on frequency and weighing)
examinations performed: no data
Necropsy performed: no data

Statistics:

LD50 values were calculated according to the method of Thompson (1947, Bacteriol. Rev. 11, 115) using the tables of Weil. (1952, Biometrics 8, 249).
The limits of +/- 1.96 standard deviations are presented.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

11 mL/kg bw

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

9 577 mg/kg bw

Any other information on results incl. tables

Fiducial range of LD50 (presented as +/- 1.96 standard deviations) was 7.0 - 18.3 ml/kg b.w. corresponding to 6094 - 1593 mg/kg b.w.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute oral LD50 of Norbornene was determined to be 11 ml/kg b.w. corresponding to 9577 mg/kg b.w.

Executive summary:

The acute oral toxicity of Norbornene was determined in groups of 5 male Carworth-Wistar rats, each receiving an oral dose of the test substance by gavage. Dosage range was spaced in a logarithmic spacing of a factor 2. The observation period was 14d. The LD50 was determined with a range of +/-1.96 standard deviations according to the calculation method of Thomson (1947). Overall the study was conducted similar to OECD 401 (retracted)

The acute oral LD50 was 9577 mg/kg b.w. in male rats (Smyth, 1969)