A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide

Long-term toxicity to fish

Administrative data

Endpoint:

adult fish: sub(lethal) effects

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

Deviations:

yes

Remarks:

28 days duration

Principles of method if other than guideline:

The draft OECD guideline proposal for "fish - juvenile growth test - 28 days" (1992) was also considered. Accordingly, the growth parameters body weight and length were included.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide

EC Number:

406-640-0

EC Name:

A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide

Cas Number:

136920-07-5

Molecular formula:

C58H114N4O6 - C154H308N6O4

IUPAC Name:

Reaction mass of 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C12-C18)alkylacetamide and {[2-(Carboxymethyl-di(C12-C18)alkylcarbamoylmethyl-amino)-ethyl]-di(C12-C18)alkylcarbamoylmethyl-amino}-acetic acid

Constituent 2

Reference substance name:

Keroflux ES 3241

IUPAC Name:

Keroflux ES 3241

Details on test material:

Test Substance No.: 90/763
Batch No.: Partie 1
Water solubility: <= 0.05 mg/L (Analytical Report ZAX 94L00436, 28 Jul 1994)

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

As the test substance is practically insoluble in water, a saturated solution prepared with 10 mg/L was tested compared to a control. No concentration control analysis was conducted as no appropriate analytical method could be established.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Forellenhof Fredelsloh, Trout Breeding, Moringen 1, FRG
- Age: Hatched April 4, 1995
- Length at study initiation: 5.79 cm (5.3 - 6.4)
- Weight at study initiation: 2.03 g (1.7 -2.4)

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

28 d

Test conditions

Hardness:

total hardness 2.3 - 2.4 mmol/L

Test temperature:

15 +/- 1°C

pH:

7.9 - 8.1

Dissolved oxygen:

8 - 10 mg/L which is about 80 - 100% of saturation

Nominal and measured concentrations:

Test concentration (nominal): 10 mg/L

Details on test conditions:

- Test vessel: Test aquarium (60 X 35 X 40 cm) with an overflow consist of glass
- Fill volume: 60 Liter
- Dilution water: Drinking water, purified over active carbon
Total hardness: 2.3 - 2.4 mmol/L
pH: 7.9 - 8.1
- Aeration: continuously aerated with oil-free air
- Photoperiod: 16 hours light/8 hours darkness
- Flow rate: 10 L/hour

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

>= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

28 d

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

No mortality or symptoms of intoxication were observed in the only concentration tested. Compared with the control group, there was no impairment of the mean body weight and length in the only test concentration.