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EC number: 700-144-0 | CAS number: 13115-71-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
A stock solution of 10.89 g/L was prepared by adding 5.4422 g test material to 500 mL of
Milli-RO water. Magnetic stirring for 15 minutes was applied to accelerate dissolution and to
ensure homogeneity. Volumes of the clear and colourless stock solution (pH 5.8)
corresponding to the test concentration were then added to the test media (16 mL synthetic
medium and made up to 250 mL with Milli-RO). Thereafter, 250 mL of activated sludge was
added resulting in the required concentrations
- Test concentration separation factor: combined limit/range-finding test - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Municipal sewage treatment plant: 'Waterschap Aa en Maas', 's- Hertogenbosch, The Netherlands, receiving predominantly domestic sewage
- Preparation of inoculum for exposure:
The sludge was coarsely sieved (1 mm) and allowed to settle. The sludge was allowed to settle again for 28 minutes. The supernatant was removed and ISO-medium was added twice.
The concentration of the suspended solids was determined. The batch of sludge was used one day after collection. Therefore, 50 mL of synthetic medium was added per liter of activated
sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Initial biomass concentration: The concentration of suspended solids was 3 g/L. - Test type:
- static
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20-21°C
- pH:
- 7.0 - 8.4
- Nominal and measured concentrations:
- Limit test: 3 replicates at 1089 mg Glycyl-L-Glutamine monohydrate/L corresponding to 1000 mg Glycyl-L-Glutamine/L (nominal)
Range finding: 1 replicate at 10.89 and 108.9 mg/L (nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass vessels
- Type (delete if not applicable): open
- Aeration: yes, clean, oil-free air; The aeration will be adjusted in such a way that the dissolved
oxygen concentration at the start is above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C) and to maintain the sludge flocs in suspension.
- No. of vessels per concentration (replicates): 3 replicates at 1089 mg/L, 1 replicate at 10.89 and 108.9 mg/L
- No. of vessels per control (replicates): 6
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 1.575 g suspended solids/L in the final test mixture
- Nitrification inhibitor used (delete if not applicable): none
TEST MEDIUM / WATER PARAMETERS
- Medium:
Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, BerkelEnschot, The Netherlands) with the following composition:
CaCl2.2H2O 211.5 mg/L
MgSO4.7H2O 88.8 mg/L
NaHCO3 46.7 mg/L
KCl 4.2 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 089 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 089 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- The EC50 of 3,5-dichlorophenol was in the accepted range of 2 to 25 mg/L for total respiration (actual value: 7.4 mg/L).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item was not toxic to waste water bacteria (activated sludge) at or below a concentration of 1089 mg/L corresponding to 1000 mg/L active ingredient (NOEC).
Reference
The mean control oxygen uptake rate exceeded 20 mg oxygen per gram of activated sludge (dry weight of suspended solids) per hour (actual value: 40 mg O2/g∙h).
The coefficient of variation of oxygen uptake in control replicates did not exceed 30 % at the end of the test (actual value: 12 %).
The EC50 of 3,5-dichlorophenol was in the accepted range of 2 to 25 mg/L for total respiration (actual value: 7.4 mg/L).
Since all validity criteria were satisfied the study was considered to be valid.
Description of key information
NOEC (3 h) > 1000 mg a.i./L (nominal, OECD 209)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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