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EC number: 610-522-6 | CAS number: 503155-49-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two short term toxicity to fish studies as-
WoE 2. and WoE 3. - GLP compliance:
- not specified
- Analytical monitoring:
- no
- Remarks:
- WoE 2 and WoE 3: no
- Vehicle:
- no
- Remarks:
- WoE 2 and WoE 3: no
- Details on test solutions:
- WoE 2: The test chemical concentrations taken foe the study were 0, 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L,100 mg/L, respectively.
WoE 3: The test solution was prepared by dissolving 400 mg of the test chemical in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- WoE 2:
TEST ORGANISM
- Common name: Zebra Fish
- Strain:Danio rerio
- Length at study initiation (length definition, mean, range and SD): average 1.7 cm
- Weight at study initiation (mean and range, SD): average 0.066 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
WoE 3:
TEST ORGANISM
- Common name:Zebra Fish
- Strain: Danio rerio
- Length at study initiation (length definition, mean, range and SD): average 3.42 cm
- Weight at study initiation (mean and range, SD): average 0.335 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- WoE 2: 150.5 mg of CaCO3
WoE 3: 140.5 mg of CaCO3 - Test temperature:
- WoE 2: 24°C
WoE 3: 25°C - pH:
- WoE 2: 7.2
WoE 3: 7.14 - Dissolved oxygen:
- WoE 2: 6.9 mg/l
WoE 3: 6.66 mg/l - Nominal and measured concentrations:
- WoE 2: Test chemical concentrations used for the study were 0, 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L,100 mg/L, respectively.
WoE 3: Nominal concentration - 100 mg/l - Details on test conditions:
- WoE 2 and WoE 3:
TEST SYSTEM
- Test vessel: Glass aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
- Reference substance (positive control):
- no
- Remarks:
- WoE 2 and WoE 3: no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: WoE 2 and WoE 3
- Sublethal observations / clinical signs:
WoE 2:
OBSERVATIONS:
Mortality and symptoms
Nominal
Concentration
(mg/l)
Number of fish
Mortality (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
6.25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
12.5
4
4
-
-
-
-
-
-
-
-
-
-
-
-
25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
50
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms
Nominal
Concentration
(mg/l)
Number of fish
symptom (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
6.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
12.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
50
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
E: Exopthalamos
pH and oxygen content
Nominal
Concentration
(mg/l)
Number of
fish
pH readings after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
7.4
7.4
7.5
7.5
7.7
7.7
7.8
7.8
6.25
4
4
7.6
7.6
7.8
7.8
7.9
7.9
7.9
7.9
12.5
4
4
7.8
7.8
7.8
7.8
8.0
8.0
8.1
8.1
25
4
4
7.8
7.8
7.9
7.9
8.1
8.1
8.2
8.2
50
4
4
7.9
7.9
8.0
8.0
8.1
8.1
8.2
8.2
100
4
4
7.9
7.9
8.0
8.0
8.1
8.1
8.2
8.2
Nominal
Concentration
(mg/l)
Number of
fish
Oxygen content (mg/L) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
6.9
6.9
6.8
6.8
6.5
6.5
6.4
6.4
6.25
4
4
6.6
6.6
6.4
6.4
6.2
6.2
6.1
6.1
12.5
4
4
6.6
6.6
6.4
6.4
6.2
6.2
6.1
6.1
25
4
4
6.5
6.5
6.4
6.4
6.2
6.2
6.0
6.0
50
4
4
6.5
6.5
6.4
6.4
6.1
6.1
6.0
6.0
100
4
4
6.5
6.5
6.4
6.4
6.1
6.1
6.0
6.0
Test temperature
Nominal
Concentration
(mg/l)
Number of
fish
Temperature (°C) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
23.2
23.2
24.1
24.1
23.7
23.7
23.5
23.5
6.25
4
4
23.2
23.2
24.1
24.1
23.7
23.7
23.5
23.5
12.5
4
4
23.2
23.2
24.1
24.1
23.7
23.7
23.5
23.5
25
4
4
23.2
23.2
24.1
24.1
23.7
23.7
23.5
23.5
50
4
4
23.2
23.2
24.1
24.1
23.7
23.7
23.5
23.5
100
4
4
23.2
23.2
24.1
24.1
23.7
23.7
23.5
23.5
WoE 3:
Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3h
6h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
symptom (cumulated) after
3h
6h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
pH and oxygen content:
NominalConcentration
(mg/l)
Number of fish
pH readings after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
7.26
7.27
7.41
7.46
8.11
8.12
8.17
8.19
100
4
4
7.71
7.22
7.81
7.82
7.96
7.97
8.13
8.19
NominalConcentration
(mg/l)
Number of fish
Temperature (°C) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
7.1
7.5
7.1
7.6
6.9
6.3
5.4
5.4
100
4
4
6.8
6.9
7.1
7.4
6.2
6.1
6.7
6.8
Test temperature
NominalConcentration
(mg/l)
Number of fish
Oxygen content (mg/L) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
22
22
22
22
22
22
22
22
100
4
4
22
22
22
22
22
22
22
22
- Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 96 hr LC50 value of the test chemical on test fish can be expected to be > 100 mg/l.
- Executive summary:
Data available for its structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on fish.The studies are as mentioned below:
An acute toxicity study was conducted for 96 hrs for assessing the effect of test chemical on fish. The test was performed following the OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.066 g and average length of 1.7 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.0 mg/l, pH 7.4, water temperature 24°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. Test concentrations selected for the study was were 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 24°C, pH 7.2, hardness of water 150.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) value was determined to be > 100 mg/l.
Another short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.335 g and average length of 3.42 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.06 mg/l, pH 7.23, water temperature 24°C and under a photoperiod of 12:12 hr light: dark conditions, respectively. Test concentrations selected for the study was 100 mg/L. Thus, limit test was performed using 100 mg/l of test chemical concentration. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 25°C, pH 7.14, hardness of water 140.5 mg of CaCO3 and under a photoperiod of 12:12 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) value was determined to be > 100 mg/l.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 96 hr LC50 value of the test chemical on test fish can be expected to be > 100 mg/l. Thus, test chemical was considered as non-toxic to aquatic fishes and hence, considered to be not classified as per the CLP classification criteria.
Reference
Description of key information
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 96 hr LC50 value of the test chemical on test fish can be expected to be > 100 mg/l.
Key value for chemical safety assessment
Additional information
Data available for its structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on fish. The studies are as mentioned below:
An acute toxicity study was conducted for 96 hrs for assessing the effect of test chemical on fish. The test was performed following the OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.066 g and average length of 1.7 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.0 mg/l, pH 7.4, water temperature 24°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. Test concentrations selected for the study was were 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 24°C, pH 7.2, hardness of water 150.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) value was determined to be > 100 mg/l.
Another short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.335 g and average length of 3.42 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.06 mg/l, pH 7.23, water temperature 24°C and under a photoperiod of 12:12 hr light: dark conditions, respectively. Test concentrations selected for the study was 100 mg/L. Thus, limit test was performed using 100 mg/l of test chemical concentration. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 25°C, pH 7.14, hardness of water 140.5 mg of CaCO3 and under a photoperiod of 12:12 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) value was determined to be > 100 mg/l.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 96 hr LC50 value of the test chemical on test fish can be expected to be > 100 mg/l. Thus, test chemical was considered as non-toxic to aquatic fishes and hence, considered to be not classified as per the CLP classification criteria.
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