Registration Dossier
Registration Dossier
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EC number: 430-910-7 | CAS number: 13335-71-2
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 Aug - 27 Aug, 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Done under GLP and OECD methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Method C3, Directive 92/69/EEC
- GLP compliance:
- yes
- Remarks:
- OECD 30
Test material
- Reference substance name:
- -
- EC Number:
- 430-910-7
- EC Name:
- -
- Cas Number:
- 13335-71-2
- Molecular formula:
- Hill formula: C10H12O3 CAS formula: C10H12O3
- IUPAC Name:
- 2-(2,6-dimethylphenoxy)acetic acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Nominal test concentrations: 10, 18, 32, 56 and 100mg/L
Mean measured test concentrations at t=0: 9.82, 17.68, 32.02, 55.33, and 97.17mg/L
Mean measured test concentrations at t=72hrs: 10.42, 17.66, 32.63, 55.89 and 98.13mg/L
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa, Institute of FreshWater Ecology, Windermere Laboratory, Far Sawrey, Ambleside, Cumbria LA22 0LP, England.
Study design
- Test type:
- other: aeration
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23 ± 2°C
- Nominal and measured concentrations:
- Nominal test concentrations: 10, 18, 32, 56 and 100mg/L
Mean measured test concentrations at t=0: 9.82, 17.68, 32.02, 55.33, and 97.17mg/L
Mean measured test concentrations at t=72hrs: 10.42, 17.66, 32.63, 55.89 and 98.13mg/L - Details on test conditions:
- Algae were cultured for 72hrs under continuous illumination of approx. 6,000 lux in the spectral range 400 -700 nm.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- <= 87.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- <= 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- <= 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- biomass
- Details on results:
- ECr50(24h) : >100mg/L measured
ECr50(48h) : >100mg/L measured
ECr50(72h) : >100mg/L measured
ECb50(24h) : 74.48mg/L measured (95% confidence limits: 61.28 - 111.48)
ECb50(48h) : 64.17mg/L measured (95% confidence limits: 51.30 - 113.33)
ECb50(72h) : 87.18mg/L measured (95% confidence limits: 60.49 - 359.25)
NOEC b (24hr): 56mg/L measured (by ANOVA)
NOEC b (48hr): 10mg/L measured (by ANOVA)
NOEC b (72hr): <10mg/L measured (by ANOVA)
NOEC r (24hr): 56mg/L measured (by ANOVA)
NOEC r (48hr): 18mg/L measured (by ANOVA)
NOEC r (72hr): <100mg/L measured (by ANOVA)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- ECr50(24h) : >100mg/L measured
ECr50(48h) : >100mg/L measured
ECr50(72h) : >100mg/L measured
ECb50(24h) : 74.48mg/L measured (95% confidence limits: 61.28 - 111.48)
ECb50(48h) : 64.17mg/L measured (95% confidence limits: 51.30 - 113.33)
ECb50(72h) : 87.18mg/L measured (95% confidence limits: 60.49 - 359.25)
NOEC b (24hr): 56mg/L measured (by ANOVA)
NOEC b (48hr): 10mg/L measured (by ANOVA)
NOEC b (72hr): <10mg/L measured (by ANOVA)
NOEC r (24hr): 56mg/L measured (by ANOVA)
NOEC r (48hr): 18mg/L measured (by ANOVA)
NOEC r (72hr): <100mg/L measured (by ANOVA)
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