1,3-Propanediol, 2,2-bis(hydroxymethyl)-, allyl ether

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 April 1996 - 18 April 1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

NA

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

Not applicable

Vehicle:

no

Details on test solutions:

The substance was dispersed in 5 L dilution water for the fish study. The mixtures were stirred with a magnetstirrer for 48 hours. These solutions were used as stock solutions (100 mg/L) for acute toxicity testing. New solutions were prepared daily.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Fish were imported and received in the laboratory on 18 February 1997 and were placed in internal quarantine for 14 days prior to the commencement of the test.

Test type:

semi-static

Water media type:

freshwater

Remarks:

Dilution water as prepared in reverse osmosis water following the ISO 6341-1982 standard as detailed on the OECD 203 test guideline.

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

Not applicable

Post exposure observation period:

Not applicable

Hardness:

250 ±25 mg CaCO3/L

Test temperature:

22 ±1°C

pH:

Range between 7.7 - 8.1 over the study duration

Dissolved oxygen:

Range between 76 - 100 % air saturation values over the study duration

Salinity:

Not applicable

Conductivity:

Not applicable

Nominal and measured concentrations:

0 (control), 5, 10, 20, 40, 80 mg/L (nominal concentration)

Details on test conditions:

TEST SYSTEM
- Test vessel: 5 L glass aquaria
- Type (delete if not applicable): open
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.2-0.3 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: based on ISO 6341-1982 standard as detailed on the OECD 203 test guideline.
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hour light / 8 hour dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): daily mortality observations

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

52 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Details on results:

The fish in the 40 mg/L treatment were markedly stressed during the 96 hour experiment which could be observed in a very high ventilation rate and hyperactivity. The 96 hour LC50 was graphically calculated to be 52 mg/L. No mortality and no behavioural differences compared to the control group were observed at 20 mg/L during the 96 hour exposure period.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

96 hour LC50 was graphically calculated.

Sublethal observations / clinical signs:

Mortality results

Nominal conc. (mg/L)	Cumulative mortality (five fish per vessel)					% mortality at 96 hours
	6 hours	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0	0	0
Control	0	0	0	0	0
5	0	0	0	0	0	0
5	0	0	0	0	0
10	0	0	0	0	0	0
10	0	0	0	0	0
20	0	0	0	0	0	0
20	0	0	0	0	0
40	0	0	1	2	2	20
40	0	0	0	0	0
80	0	5	5	5	5	100
80	2	5	5	5	5

 

Validity criteria fulfilled:

yes

Conclusions:

The fish in the 40 mg/L treatment were markedly stressed during the 96 hour experiment which could be observed in a very high ventilation rate and hyperactivity. The 96 hour LC50 was graphically calculated to be 52 mg/L. No mortality and no behavioural differences compared to the control group were observed at 20 mg/L during the 96 hour exposure period.

Executive summary:

The acute toxicity of 1,3-propanediol, 2,2- bis(hydroxymethyl) -, allyl ether to Zebra fish was determined. The study was carried out according to OECD test guideline 203 - Acute Toxicity for Fish. In this study Zebra fish were exposed to the test material over a test period of 96 hours under semi-static conditions with test media renewal every 24 hours. The test material was dispersed in 5 L dilution water for the fish study. The mixtures were stirred for 48 hours. These solutions were used as stock solutions (100 mg/L) for acute toxicity testing. New test solutions were prepared daily.

Nominal exposure concentrations were 0 (control), 5, 10, 20, 40 and 80 mg/L.  The results of the study were based on the nominal concentrations.

The fish in the 40 mg/L treatment were markedly stressed during the 96 hour experiment which could be observed in a very high ventilation rate and hyperactivity. The 96 hour LC₅₀was graphically calculated to be 52 mg/L and the corresponding NOEC, based on mortality and behavioural differences was observed to be 20 mg/L.

Description of key information

The 96 hour LC₅₀was graphically calculated to be 52 mg/L and the corresponding NOEC, based on mortality and behavioural differences was observed to be 20 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

52 mg/L

Additional information

The acute toxicity of 1,3-propanediol, 2,2- bis(hydroxymethyl) -, allyl ether to Zebra fish was determined. The study was carried out according to OECD test guideline 203 - Acute Toxicity for Fish. In this study Zebra fish were exposed to the test material over a test period of 96 hours under semi-static conditions with test media renewal every 24 hours. The test material was dispersed in 5 L dilution water for the fish study. The mixtures were stirred for 48 hours. These solutions were used as stock solutions (100 mg/L) for acute toxicity testing. New test solutions were prepared daily.

Nominal exposure concentrations were 0 (control), 5, 10, 20, 40 and 80 mg/L.  The results of the study were based on the nominal concentrations.

The fish in the 40 mg/L treatment were markedly stressed during the 96 hour experiment which could be observed in a very high ventilation rate and hyperactivity. The 96 hour LC₅₀was graphically calculated to be 52 mg/L and the corresponding NOEC, based on mortality and behavioural differences was observed to be 20 mg/L.