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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not applicable
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Test item concentration:
- the test item was added to test medium at a nominal concentration of 100 mg/L
- The test substance tended to float on the water surface and was therefore shaken vigorously with a vortex apparatus and treated with ultrasonic waves for 5 minutes
- Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test
substance
- After stirring the 100 mg/L solution appeared to be a greyish green turbid dispersion with a floating layer; the solution was used as such.
Test organisms (species):
Cyprinus carpio
Details on test organisms:
- Source : Zodiac, proefacc, "De Haar Vissen", L.U., Wageningen, the Netherlands
- Mean length: 2.26 ± 0.19 cm
- Mean weight: 0.23 ± 0.06 g
- Characteristics: F1 from a single parent-pair bred in UV-treated water
- Reason for selection: This system has been selected as an internationally accepted species
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
218 mg/L CaCO3)
Test temperature:
19.6-21.6°C
pH:
7.6-7.9
Dissolved oxygen:
7.6-8.3 (mg/L)

Salinity:
n.a.
Conductivity:
no data
Nominal and measured concentrations:
100 mg/L test item
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Remarks:
(mortality in the controls < 10% by the end of the test; dissolved oxygen concentration above 60% of the air-saturation value throughout the exposure period; pH did not vary by more than 1 unit)
Conclusions:
Under the conditions of the present test Solvent green 28 induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/L during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/L, the regulatory limit concentration.
Executive summary:

The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992). Under the conditions of the present test Solvent green 28 induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/L during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/L, the regulatory limit concentration. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Fish study.

Description of key information

Under the conditions of the present test Solvent green 28 induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/L during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/L, the regulatory limit concentration.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

"Should read: >100 mg/L"