Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-338-6
CAS number: 94-49-5
results: The results showed that the arithmetic mean concentrations in
0.181, 0.353, 0.726 and 1.429 mg/L, which were 61.4% ~ 69.0% of the
nominal concentration, the determined concentrations in old media were
significantly decreased and many values were lower than LOQ or no signal
mean exposure concentration over the whole period was calculated from
the geometric mean concentration of each renewal period (daily). For the
nominal concentrations of 0.144, 0.288, 0.575, 1.15 and 2.3 mg/L, the
mean exposure concentrations were 0.035, 0.045, 0.073, 0.124 and 0.345
Fish, Early-life Stage
Ethylene glycol dibenzoate was conducted withzebra
rerio) in our facility.
Based on the results of
a preliminary test, the definitive test was conducted at 5 nominal
concentrations of 0.144, 0.288, 0.575, 1.15 and 2.3 mg/L, concurrently
with a blank control group. 80 fertilised eggs were used for test
concentrations and control and divided equally between 4 replicate test
The test was performed
with a semi-static system andfrequency
of solution renewalwas
organisms were retainedin
the beakers whilst 4/5 of test solution/control volume was changed.
During the exposure
period, observations on hatching and survival were made one time on 3
hours after exposure and then daily and the number were recorded.For
embryos, larvae and juvenile fish,theabnormal
appearance or behaviourobserved
were also recorded. The dry weightson
a replicate basisand
individual total lengths were measured at the end of the test.
The concentrations of
test item were determined in samples of test media by HPLC method.
During the test, the concentrations of the test chemical were analysedon
two occasions perweek,
in fresh and old media(i.e.
analyses were made on a sample from the same solution when freshly
prepared and at renewal). The mean exposure concentration over the whole
period was calculated from the geometric mean concentration of each
renewal period (daily). For the nominal concentrations of 0.144, 0.288,
0.575, 1.15 and 2.3 mg/L, the mean exposure concentrations were 0.035,
0.045, 0.073, 0.124 and 0.345 mg/L.
At the control and
measured concentrations of 0.035, 0.045, 0.073 mg/L, there were noabnormal
appearance or behaviourobserved
larvae. At the
measured concentrations of 0.124 and 0.345 mg/L, theabnormal
appearances such as curved body and yolk sac oedema were observedonembryos,theabnormal
appearances or behaviour such as curved body, pericardial oedema,
atypical quiescence and uncoordinated swimming were observed on larvae.
Hatching rate and
survival rate of post-hatch success, abnormal appearance or behaviourofembryos
dry weightson a
individual total lengths,days
to begin of hatch, 90% hatching and end of hatchingwere
calculated. The data wereusedfor
statistical analysis usingTwo-sidedStudentt-test
with a 5% significance level. Probit analysis (EPA Version 1.5) was used
to calculate LC50of embryo and larvae and EC50of
abnormal appearance or behaviour of embryo and larvae.
The NOEC/LOEC and LC50/
EC50for biological endpoints are as follows, based onthegeometricmeanconcentration
LC50/EC50and 95% confidence limits (mg/L)
Hatching rate (embryo survival)
Survival rate of post-hatch success (larvae survival)
Growth at the end of test:
Dry weightson a replicate basis
Individual total lengths
Other observed effects:
Abnormal appearance or behaviouroflarvae
0.126 (95% confidence limits was not obtained)
Day to begin of hatch
Day of 90% hatching
Day of end of hatching
long-term fish study according to OECD 210 guideline under GLP was
conducted with Zebra
test was performed with a semi-static system, and test solutions were
renewed every 24 hours.
the exposure period, observations on hatching and survival were made one
time on 3 hours after exposure and then daily and the number were
recorded. For embryos,
larvae and juvenile fish, the abnormal appearance or behaviour observed
were also recorded. The dry weights on a replicate basis and individual
total lengths were measured at the end of the test.
geometric mean exposure concentration were calculated and used for
NOEC/LOEC and EC50/LC50for biological endpoints
are as follows, based on the
EC50/LC50and 95% confidence limits (mg/L)
dry weights on a replicate basis
individual total lengths
Abnormal appearance of embryos
Abnormal appearance or behaviour of larvae
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again