1-amino-4-(ethylamino)-9,10-dihydro-9,10-dioxoanthracene-2-carbonitrile

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

not specified

Test temperature:

35 °C

Details on study design: HPLC method:

Stock solutions of the reference substance at concentrations of 1000 mg/L in methanol were used. 10 µL of the stock solution was within the 390 µL tetrahydrofuran and 400 µL of 0.1 M phosphate buffer pH 7. The blank solution for the mixture of reference substances was 1/3960 (v/v) methanol/tetrahydrofuran /0,1M phosphate buffer pH 7.
A 1000 mg/L stock solution of the test item was prepared in tetrahydrofuran. The stock solution and the solutions of reference substances were diluted to obtain an end solution of 40/60 (v/v) tetrahydrofuran/0.1M phosphate buffer pH 7. The final concentration of the test item was 10.0 mg/L. The test item blank solution was 40/60 (v/v) tetrahydrofuran/0.1M phosphate buffer pH 7.
The reference substance and test item solutions were injected in duplicate. Blank solutions were analysed by single injection.

Key result

Type:

Koc

Value:

ca. 4 200 dimensionless

pH:

7

Temp.:

35 °C

Type:

log Koc

Value:

ca. 3.62 dimensionless

pH:

7

Temp.:

35 °C

Details on results (HPLC method):

The UPLC-UV chromatograms for the test item solution showed a single test item peak. The Koc and logKoc values of the test item at pH 7 were 4.2x103 and 3.62 respectively.

Conclusions:

The HPLC method using soil-adsorption-reference was applied for the determination of the adsorption coefficient (Koc) of Disperse Blue 359. The Koc and logKoc values of the test item at pH 7 were 4.2x103 and 3.62 respectively.

Executive summary:

The HPLC method using soil-adsorption-reference was applied for the determination of the adsorption coefficient (K_oc) of Disperse Blue 359. The K_ocand logK_ocvalues of the test item at pH 7 were 4.2x10³and 3.62 respectively.

Description of key information

The HPLC method using soil-adsorption-reference was applied for the determination of the adsorption coefficient (K_oc) of Disperse Blue 359. The K_ocand logK_ocvalues of the test item at pH 7 were 4.2x10³and 3.62 respectively.

Key value for chemical safety assessment

Koc at 20 °C:

4 200

Additional information

The HPLC method using soil-adsorption-reference was applied for the determination of the adsorption coefficient (K_oc) of Disperse Blue 359. The K_ocand logK_ocvalues of the test item at pH 7 were 4.2x10³and 3.62 respectively.

[LogKoc: 3.62]