Tin sulphide

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-08-29 - 2012-12-07

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

no GLP

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.6200 (Earthworm Subchronic Toxicity Test)

Version / remarks:

1996

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)

Deviations:

no

GLP compliance:

no

Remarks:

Test performed in China

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name:
- Source: Earhworm nursery of Nanjing city in China
- Age at test initiation (mean and range, SD): adult (two months with clitellum)
- Weight at test initiation (mean and range, SD): 319 mg
- Batch No.: E201210815

ACCLIMATION
- Acclimation period:24 days in non polluted soil and then for 24 h in test soils

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

20 +- 2°C

pH:

6.0 - 6.3

Moisture:

35%

Details on test conditions:

TEST SYSTEM

- Amount of soil or substrate: 750 g per container
- No. of organisms per container (treatment):10
- No. of replicates per treatment group: 4
- No. of replicates per control: 1


Artificial Soil:
10% sphagnum peat
20% kaolin clay
70% industrial sand (60% of particles between 50 and 200 microns)
-> mixed thoroughly

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 400 - 800 lux
- Humidity: 80-85-%

TEST CONCENTRATIONS
- Range finding study: observed concentrations 10, 100 and 1000 mg/kg artifical soil (dry weight)
- Results used to determine the conditions for the definitive study: no mortality was observed in highest concetration of Range-Finding-Test, highest tested concentration was used for limited test
- Test concentrations: limited test with 1000 mg/kg artifical soil (dry weight)

On 7th day of experiment worms were sorting from the medium onto a stainless plate. Reactions to a mechanical stimulus, behavioral and pathological symptoms were observed. After that soils were replaced in the test container and survived earthworms were replaced on same test substrate surface.

Nominal and measured concentrations:

nominal concentrations Range-Finding-Test: 10, 100, 1000 mg/kg soil dry weight
nominal concentrations Limit Test: 1000 mg/kg soil dry weight

Reference substance (positive control):

yes

Remarks:

2-Chloracetamide

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Basis for effect:

mortality

Results with reference substance (positive control):

Test conditions were adaquate.

Reported statistics and error estimates:

Validity of Test:
- mortality in untreated control group must not exceed 10%
- reference control to asure adequate test conditions

LC50 and 95% confidence limits were calculated by Trimmed Spearman-Karber Method. No statistical method was used, because of low toxicity of test substance on earthworm.

Validity criteria fulfilled:

yes

Conclusions:

Test results indicated that the 14 d LC50 of test item in artifical soil was greater than 1000 mg/kg dry weight soil. NOEC after 14d was greater than 1000 mg/kg dry weight soil.

Executive summary:

The acute toxicity of tin sulfide on earthworm Eisenia fetida was determined in accordance OECD Guideline 207 "Earthworm, Acute Toxicity Tests". Therefore 10 organism/ replicate (4) were exposed in samples of artificial soil mixed with test item. Mortality was assessed 7 days and 14 days after application of test item (1000 mg/kg dry weigth soil). Based on the results of Limit Test the 14 d NOEC and LC50 were determined and be greater than 1000 mg tin sulfide/kg dry weigth soil. Therefore, tin sulfide has low acute toxicity to earthworms.

Description of key information

Earthworm 14 day short-term test:

NOEC >= 1000 mg/kg dw

LC50 > 1000 mg/kg dw

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw

Additional information

The acute toxicity of tin sulfide on earthworm Eisenia foetida was determined in accordance OECD Guideline 207 "Earthworm, Acute Toxicity Tests". Therefore 10 organism/ replicate (4) were exposed in samples of artificial soil mixed with test item. Mortality was assessed 7 days and 14 days after application of test item (1000 mg/kg dry weigth soil). Based on the results of Limit Test the 14 d NOEC and LC50 were determined and be greater than 1000 mg tin sulfide/kg dry weigth soil. Therefore, tin sulfide has low toxicity to earthworms.

No further testing on soil macroorganisms exept arthopods is required because the substance ist not considered hazardous and there is no indication of an accumulation and toxicity to soil organisms.