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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
4 March, 1996 -15 March, 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
not specified
Specific details on test material used for the study:
Description Colourless liquid
Batch 41067148
Purity Not indicated by sponsor; treated as 100% pure
Test substance storage At room temperature in the dark
Stability under storage conditions Stable
Expiry date September 14, 1998
Stability in vehicle Water: at least 96 h
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
The test solutions were prepared directly in ISO-medium using ultra-sonic treatment for 5 minutes.
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, the Netherlands.
Mean length: 1.95 + 0.23 cm
Mean weight: 0.27 + 0.04 g
Total fish used: 65
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no
Hardness:
After aeration the hardness was 250 mg CaCÛ3 per litre and the pH was 8.0 +/- 0.2.
Test temperature:
20.6 degree C +/- 2 degrees
pH:
pH 8.0 +/- 2
Dissolved oxygen:
9.2 +/- 8
Nominal and measured concentrations:
10, 18, 32, 56, and 100 mg/l
Details on test conditions:
Test duration: 96 hours
Test type: Static
Test vessels: 4 litres, all-glass.
Test medium: ISO-medium, aerated until the dissolved oxygen concentration had reached saturation and the
pH had stabilized. After aeration the hardness was 250 mg CaCO3 per litre and the pH was 8.0 +/- 0.2.
Number of fish: 7 fish per concentration and controls.
Loading: 0.95 g fish/litre of test media
Illumination: 16 hours photoperiod daily
Aeration: The test media were aerated continuously.
Feeding: No feeding from 24 hours prior to the test and during the total test period.
Introduction of fish: Directly after preparation of the test media
Euthanasia: At the end of the test the surviving fish were rapidly killed by exposing-them to ca. 1.2% ethylene glycol monophenylether in water.
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
75 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Validity criteria fulfilled:
yes
Conclusions:
The 96h-LC50 for carp is 75 mg/l with 0 % mortality at 56 mg/l and 100 % mortality at 100 mg/l.
Executive summary:

The study procedures described in this report were based on the EEC directive 92/69, Part C.l. "Acute toxicity for fish"; and the OECD guideline No. 203: "Fish Acute Toxicity Test", Adopted 17 July, 1992. The definitive test was performed with carp exposed to concentrations ranging from 10 to 100 mg/l in a static system. The 96h-LC50 for carp is 75 mg/l with 0 % mortality at 56 mg/l and 100 % mortality at 100 mg/l.

Description of key information

The study procedures described in this report were based on the EEC directive 92/69, Part C.l. "Acute toxicity for fish"; and the OECD guideline No. 203: "Fish Acute Toxicity Test", Adopted 17 July, 1992. The definitive test was performed with carp exposed to concentrations ranging from 10 to 100 mg/l in a static system. The 96h-LC50 for carp is 75 mg/l with 0 % mortality at 56 mg/l and 100 % mortality at 100 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
75 mg/L

Additional information