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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
48 hours
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Purity: >90%
Batch: 900150909STG115
Analytical monitoring:
yes
Details on sampling:
Chemical analysis of the test item was carried out at 0 and 48 hours.
Vehicle:
yes
Remarks:
dimethylformamide
Details on test solutions:
A nominal amount of test item (100 mg) was dissolved in dimethylformamide and the volume adjusted to 10 mL to give a 100 mg/10 mL solvent stock solution. An aliquot (500 μL) of this 100 mg/10 mL solvent stock solution was dispersed in 5 liters of deionized reverse osmosis water with the aid of magnetic stirring for approximately 30 minutes to give the required test concentration of 1.0 mg/L. After stirring any undissolved test item was removed by centrifugation at 10000 g for 30 minutes to give a 100% v/v solution of the test item.
Test organisms (species):
Daphnia magna
Details on test organisms:
1st instar Daphnia magna derived from in-house laboratory cultures
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Hardness:
The reconstituted water had an approximate theoretical total hardness of 250 mg/L as CaCO3
Test temperature:
21-22 deg C
pH:
7.8 +/- 0.2
Dissolved oxygen:
8.7-9 mg O2/L
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.042 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Nominal
Concentration
(% v/v)

Observations (Initial Population: 10 Per Replicate)

24 Hours

48 Hours

Cumulative Immobilized Daphnia

Observations

Cumulative Immobilized Daphnia

Observations

Control

0

10 N

0

10 N

Solvent Control

0

10 N

0

10 N

0.10

0

10 N

0

10 N

1.0

0

10 N

0

10 N

10

0

10 N

0

10 N

100

0

10 N

7

3 N

Validity criteria fulfilled:
yes
Conclusions:
Exposure of Daphnia magna to the test item was investigated and gave the following result, EC50 0.042 mg/L.
Executive summary:

The substance was tested following the methods outline in the OECD 202 guideline. The test was conducted to GLP standards and fulfilled the validity criteria. The exposure of Daphnia magna to the test item gave an EC50 of 0.042 mg/L. As this result is above the water solubility of the substance, the substance is not considered toxic.

Description of key information

A 48h-EC50 of 0.042 mg/L was determined following the OECD 202 guidelines. This result is above the water solubility therefore the substance is not classified as acutely toxic. 

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.042 mg/L

Additional information

The available studies on the short-term toxicity of IDDPP towards aquatic invertebrates are listed in the table below:

 

Guideline

Test species

Endpoint

Result

Remarks

EPA-660/3-75-009

Daphnia magna

48h-EC50

0.34 mg/l (nominal) Pydraul 312C

Supporting study, rel. 2

US EPA, 1975. Methods for acute toxicity tests with fish, macroinvertebrates and amphibians.

Chironomus tentans

48h-EC50

0.61 mg/l (nominal) Santicizer 148

Supporting study, rel. 2

US EPA, 1975. Methods for acute toxicity tests with fish, macroinvertebrates and amphibians.

Paratanytarsus parthenogenetica

48h-EC50

1.32 mg/l (nominal) Santicizer 148

Supporting study, rel. 2

48h-EC50

Supporting study, rel. 3

Daphnia sp., 48 -hour Acute Immobilization test OECD 202, Envigo 2016

 Daphnia magna

  

48h-EC50

 0.042 mg/L (geomean measured)

 Key Study, GLP, rel. 1

As all measured toxicity values are well above the water solubility (11.2 ug/l) of the substance, IDDPP can not be classified as toxic to the environment.