3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarbonyl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data is from publication.

Data source

Reference

Reference Type:

publication

Title:

Genetic Toxicity study for Cyclohexene by Bacterial reverse mutation assay

Author:

National Institute of Technology and Evaluation

Year:

2017

Bibliographic source:

Japan chemicals collaborative knowledge database (J-check), 2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Guidelines for Screening Mutagenicity Testing of Chemicals (Chemical Substances Control Law of Japan) and OECD Test Guideline 471

Principles of method if other than guideline:

To evaluate the mutagenic potential of Cyclohexene in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia
coli WP2 uvrA.

GLP compliance:

not specified

Type of assay:

bacterial forward mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexene

EC Number:

203-807-8

EC Name:

Cyclohexene

Cas Number:

110-83-8

Molecular formula:

C6H10

IUPAC Name:

Cyclohexene

Details on test material:

Details on test material
- Name of test material (as cited in study report): Cyclohexene
Molecular form; C6H10

Specific details on test material used for the study:

Details on test material
- Name of test material (as cited in study report): Cyclohexene
Molecular form; C6H10
Purity: 98.63 wt%

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA.

Details on mammalian cell type (if applicable):

Not applicable.

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

not specified

Metabolic activation:

with and without

Metabolic activation system:

Rat liver, induced with phenobarbital and 5, 6-benzoflavone

Test concentrations with justification for top dose:

-S9 mix; 0, 19.5, 39.1, 78.1, 156, 313, 625, 1250 mg/plate (TA100,TA1535, TA98, TA1537)
-S9 mix; 0, 78.1, 156, 313, 625, 1250, 2500, 5000 mg/plate (WP2 uvrA)
+S9 mix; 0, 19.5, 39.1, 78.1, 156, 313, 625, 1250 mg/plate (all strains)

Vehicle / solvent:

Vehicle
- Vehicle(s)/solvent(s) used: Ethanol

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

Remarks:

Ethanol

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

sodium azide

other: -S9 mix; 2-(2-Furyl)-3-(5-nitro-2-furyl) acrylamide (TA100, TA98 and WP2 uvrA), 2-Methoxy-6-chloro-9-[3-(2- chloroethyl)-aminopropylamino] acridine¡¦2HCl (TA1537) ,+S9 mix; Benzo[a]pyrene (TA100, TA98, TA1537) and 2- Aminoanthracene (TA1535 and WP2 uvrA)

Details on test system and experimental conditions:

Details on test system and conditions
METHOD OF APPLICATION: Pre-incubation method

Other: 3 plates per test were observed.

Rationale for test conditions:

Not specified.

Evaluation criteria:

To evaluate the histidine revertant /plate

Statistics:

Not specified

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA.

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

Not specified.

Remarks on result:

other: No mutagenic effect were observed.

Applicant's summary and conclusion

Conclusions:

Cyclohexene (110-83-8) was evaluated for its mutagenic potential in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA. The test result was considered to be negative in the presence and absence of metabolic activation.

Executive summary:

Cyclohexene was assessed for its possible mutagenic potential. For this purpose Bacterial reverse mutation assay was performed according toGuidelines for Screening Mutagenicity Testing of Chemicals (Chemical Substances Control Law of Japan) and OECD Test Guideline 471 on Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichiacoli WP2 uvrA. The test material was exposed in the presence and absence of S9 at different concentration. No mutagenic effects were observed in any strain. Therefore Cyclohexene was considered to be non mutagenic in the presence and absence of metabolic activation. Hence test substance cannot be classified as gene mutant in vitro.