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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
OECD 202
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
other: The Notice on the Test Method Concerning New Chemical Substances
Deviations:
no
GLP compliance:
yes
Test material information:
Composition 1
Analytical monitoring:
yes
Vehicle:
not specified
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test water.
Test organisms (species):
Daphnia sp.
Details on test organisms:
TEST ORGANISM
- Feeding during test: No
Test type:
semi-static
Water media type:
other: dechlorinated tap water
Limit test:
no
Total exposure duration:
48 h
Test temperature:
19.9 - 20.1 ºC
Nominal and measured concentrations:
Nominal:30, 20, 13, 8.9, 5.9 mg/L,(geometric ratio 1.5) control
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume: Test solution volume 400 mL/concentration (100 mL/vessel)
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): After 24 hours of exposure
- No. of organisms per vessel: 5 (20 /concentration)
- No. of vessels per concentration (replicates):4

OTHER TEST CONDITIONS
- Photoperiod: Room light
- Light intensity: 16 hours light / 8 hours dark
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 12 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
mobility
Remarks on result:
other: 95% CL= 10 - 14mg/L
Details on results:
- Lowest concentration causing 100 % immobility (48 h): > 30 mg/L
- Highest concentration causing 0 % immobility (48 h): 5.9 mg/L
All results were based on the nominal values.
Validity criteria fulfilled:
not specified
Conclusions:
The test item has effective concentration EC50 (48h) of 12mg/L . (Acute toxicity on daphnia magna)
Executive summary:

Based on available data from National instititute of technology and evaluation (NITE) test item was tested to the toxicity to freswater species of Daphnia Magna, in the nominal concentration: 30, 20, 13, 8.9, 5.9 mg/L , 0 mg/L (control) and the temperature 19.9 - 20.1 ºC according the OECD TG 202, followed GLP principles. Test was performed in semi-static condition during 48 hours. Five organism per vessels (20 per concentrations) were used. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test water.The Effective concentration EC50 (48h) of 12 mg/L based on mobility was evaluated.

Description of key information

Key study: Based on available data from National instititute of technology and evaluation (NITE) test item has Effective concentration EC50 (48h) of 12 mg/L based on mortality.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
12 mg/L

Additional information

Key study:OECD TG 202, followed GLP principles.

Based on available data from National instititute of technology and evaluation (NITE) test item was tested to the toxicity to freswater species of Daphnia Magna, in the nominal concentration: 30, 20, 13, 8.9, 5.9 mg/L , 0 mg/L (control) and the temperature 19.9 - 20.1 ºC . Test was performed in semi-static condition during 48 hours. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test water.The Effective concentration EC50 (48h) of 12 mg/L based on mobility was evaluated.