2-benzylideneheptanal

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from J-check authoritative databases

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Principles of method if other than guideline:

Short term toxicity study of alpha-Amyl cinnamaldehyde on algal growth inhibition test was carried out for 72hrs

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): alpha-Amyl cinnamaldehyde
- Molecular formula (if other than submission substance): C14H18O
- Molecular weight (if other than submission substance): 202.2952
- Smiles notation (if other than submission substance): c1(\C=C(\CCCCC)C=O)ccccc1
- InChl (if other than submission substance): 1S/C14H18O/c1-2-3-5-10-14(12-15)11-13-8-6-4-7-9-13/h4,6-9,11-12H,2-3,5,10H2,1H3/b14-11-
- purity- No data

Analytical monitoring:

not specified

Details on sampling:

No data

Vehicle:

not specified

Details on test solutions:

No data

Test organisms (species):

not specified

Details on test organisms:

No data

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Remarks on exposure duration:

No data

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

No data

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

Nominal

Details on test conditions:

No data

Reference substance (positive control):

not specified

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.21 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

2.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: AUG

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.66 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: AUG

Details on results:

No data

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Validity criteria fulfilled:

not specified

Conclusions:

Based on the growth rate the EC50 and the NOEC concentration was >1.5 mg/l and 0.21mg/l. and on the basis of AUG, the EC50 and NOEC concentration was 2.3mg/l and 0.66mg/l.

Executive summary:

Short term toxicity study of alpha-Amyl cinnamaldehyde to algal growth inhibition test was carried out for 72hrs on the freshwater algae. Study performed by using static method. Based on the growth rate the EC50 and the NOEC concentration was >1.5 mg/l and 0.21mg/l. And on the basis of AUG, the EC50 and NOEC concentration was 2.3mg/l and 0.66mg/l. Based on the above concentration the chemical can be considered as toxic at environmentally relevant concentrations..

Description of key information

Based on the experimental key study (Japan chemicals collaborative knowledge database (J-check), 2017), Short term toxicity study of alpha-Amyl cinnamaldehyde to algal growth inhibition test was carried out for 72hrs on the freshwater algae. Study performed by using static method. Based on the growth rate the EC50 and the NOEC concentration was >1.5 mg/l and 0.21mg/l. But on the basis of AUG, the EC50 and NOEC concentration was increases i.e. 2.3mg/l and 0.66mg/l. based on the above concentration of NOEC and EC50 the chemical alpha-Amyl cinnamaldehyde is toxic to aquatic algae at environmentally relevant concentrations.

Key value for chemical safety assessment

EC50 for freshwater algae:

2.3 mg/L

EC10 or NOEC for freshwater algae:

0.66 mg/L

Additional information

Based on the various experimental data and prediction data by using the EPI suite ECOSAR for the target chemical have been reviewed to determine the toxic nature of alpha-Amyl cinnamaldehyde (122-40-7), IUPAC name 2-benzylideneheptanal. The studies are as mentioned below:

Based on the experimental key study (Japan chemicals collaborative knowledge database (J-check), 2017), Short term toxicity study of alpha-Amyl cinnamaldehyde to algal growth inhibition test was carried out for 72hrs on the freshwater algae. Study performed by using static method. Based on the growth rate the EC50 and the NOEC concentration was >1.5 mg/l and 0.21mg/l. But on the basis of AUG, the EC50 and NOEC concentration was increases i.e. 2.3mg/l and 0.66mg/l. based on the above concentration of NOEC and EC50 the chemical alpha-Amyl cinnamaldehyde can be classified in the chronic category 2 as per the classification.

In the study (ECOSAR Version 1.11),Based on the prediction done using the EPI Suite ECOSAR version 1.11, the short term toxicity on green algae was predicted for test substance α-Amyl Cinnamaldehyde, CAS (122 -40 -7). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 1.715mg/l for Species for 96hrs duration. Based on this value, it can be concluded that the test chemical α-Amyl Cinnamaldehyde can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in chronic category 2 as per the CLP classification criteria.

 

In the supporting study (Timothy J. Ward, Derek C. Wyskiel, Robert L. Boeri, 2003) Growth and reproduction toxicity test with alpha-Amyl cinnamaldehyde on the freshwater alga,Selenastrum capricornutum was carried out for 72hrs by using static method at different concentrations. Control and stability sample was also use with the incubation period of 72hrs. Results observed at the interval of 24hrs. After 48hrs incubation EC50 was 1.82, 1.89, 1.58mg/l on the basis of no of cells, average specific growth rate, and area under the growth curve. With the 95% confidence limit of 1.38-2.41, 1.58-2.25, 1.20-2.09. But after 72hrs the EC50 value was 1.18mg/l,1.88mg/l, 1.24mg/l on the basis of no of cells, average specific growth rate, and area under the growth curve (AUG) with the 95% confidence limit of 1.03 to 1.36, 1.79 to 1.97mg/l, 1.09 to 1.41 mg/l. Based on the growth inhibition of the test Selenastrum capricornutum the 72hrs EC50 Value was 1.18mg/l, 1.88mg/l, 1.24mg/l on the basis of no of cells, average specific growth rate, and area under the growth curve (AUG). And the NOEC was 0.154mg/l. Based on the EC50 it can be concluded that the chemical alpha-Amyl cinnamaldehyde can be classified in chronic category 2 as per the CLP classification criteria.

 

In another supporting study for evaluating the toxic nature of chemical alpha-amyl cinnamaldehyde (122-40-7) (Inventory Multitiered Assessment and Prioritisation, NICNAS, 2017) Short term toxicity study to Pseudokirchneriella subcapitata was carried out by using the chemical alpha-Amyl cinnamaldehyde (IUPAC name 2-benzylideneheptanal) for 48 and 72 hrs. The experiment perform as per the OECD guideline, by using static method. Based on the growth inhibition of daphnia magna by the exposure of alpha-Amyl cinnamaldehyde the EC50 and the NOEC concentration was 1.88mg/l and 0.15mg/l. based on the above concentration the chemical classified in the chronic category 2 as per the CLP classification.

 

Thus, based on the overall reported results for target chemical α-Amyl Cinnamaldehyde, CAS (122-40 -7)andIUPAC name 2-benzylideneheptanal, from J-check, Timothy J. Ward, Derek C. Wyskiel, Robert L. Boeri, 2003, (Inventory Multitiered Assessment and Prioritisation, NICNAS, 2017 and prediction from ECOSAR version 1.11. it can be considered as toxic to aquatic environment and can be considered to be classified in chronic category 2 as per the CLP classification criteria.