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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given; test procedure according to national standards (DIN); conducted under Quality Assurance Programme; minor restrictions in design (study conducted for 48 h instead of 96 h), but otherwise adequate for assessment
Qualifier:
according to
Guideline:
other: DIN guideline 38412, part 15
Deviations:
no
GLP compliance:
yes
Remarks:
Quality Assurance Programme/GLP standards not specified
Analytical monitoring:
yes
Details on sampling:
Initial exposure levels were measured in the control and in the test solution.

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: water accommodated fraction (WAF)
A saturated solution (initial weight: 150 mg/l) was prepared in reconstituted water and was stirred overnight. After a filtration step over a folded paper filter (595 1/2; diameter 320 mm, Schleier & Schuell, D-37582 Dassel) the filtrate was used without further treatment.
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden Orfe
- Source: fish farm Eggers, Hohenwestedt, Germany
- Length at study initiation: 5-8 cm
- Corpulence factor: 0.8-1.1 g/cm3
- Feeding: Tetra Menü Spezialfutter
- Feeding during test: fish were not fed during the test

ACCLIMATION
- Acclimation period: at least 7 days
- Acclimation conditions: 20 - 21 °C
- Diluent water: tap water of Karlsruhe with the following quality:
acid capacity KS(4.3): 4.75 - 4.77 mmol/L
water hardness: 2.83 - 3.14 mmol/L
calcium Ca2+: 2.41 - 2.60 mmol/L
magnesium Mg2+: 0.42 - 0.54 mmol/L
sodium Na+: 0.28 - 0.33 mmol/L
potassium K+: 0.05 - 0.06 mmol/L
pH-value: 7.39 - 7.49
aeration: at least 80% of the air saturation value
- Type of food: Tetra Menü Spezialfutter
- Health during acclimation: fish were in good health
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
2.7 mmol CaCO3/L
Test temperature:
20.3-20.4 °C
pH:
7.96 (control)
7.15 (saturated test solution)
Dissolved oxygen:
At least 80% of the air saturation value
Nominal and measured concentrations:
nominal loading rate: 150 mg/L
TOC-values at test start:
test solution (WAF): 14.4 mg/L
control: 0.054 mg/L
Details on test conditions:
TEST SYSTEM
- Volume: 10 L
- Aeration: permanent during the test duration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 – 5.6 g/L (corpulence factor K = 0.8-1.1 g/cm³, length = 5-8 cm)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Total organic carbon: 0.054 mg/L
- Acid capacity, KS(4.3): 0.7-0.9 mmol/L
- Inorganic salts: CaCl2: 2.2 mmol/L, MgSO4: 0.5 mmol/L, NaHCO3: 0.64 mmol/L
- Ca/mg ratio: 4:1
- Culture medium different from test medium: yes

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light: 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality recorded after 48 h
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
LL50
Effect conc.:
> 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: results are expressed in terms of loading rates
Duration:
48 h
Dose descriptor:
LL100
Effect conc.:
> 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: results are expressed in terms of loading rates
Duration:
48 h
Dose descriptor:
NOELR
Effect conc.:
>= 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: results are expressed in terms of loading rates
Details on results:
In a saturated solution of the test material in dilution water no mortality of fish after 48 hours was observed. Sub-lethal effects were not reported.
Validity criteria fulfilled:
yes

Description of key information

48-h LC50 (based on loading rates)  >150 mg/L for Leuciscus idus (DIN guideline 38412, part 15)

Key value for chemical safety assessment

Additional information

LC50 for freshwater fish: > 150 mg/L

The results indicate that the test material is not toxic to fish up to the limits of its water solubility and a NOEC and thus a PNEC cannot be determined.