(aminoalkyl) hydrogen thiosulfate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 2011-12-21 to 2012-03-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Testing Methods for New Chemical Substances; No. 0331-7, Ministry of Health, Labour and Welfare; 2011.03.29, No. 5, Ministry of Economy, Trade and Industry; No. 110331009, Ministry of Environment, Japan.

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): PSF-58
- Physical state: white crystalline powder
- Analytical purity: 100%
- Lot/batch No.: KNC110310
- Stability under test conditions: stable for 2 years in ambient temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: standard activated sludge purchased from Chemicals Evaluation and Research Institute, Japan
- Laboratory culture: cultured at the test facility in 25 °C (± 2 °C), not less than 5 mg/L dissolved oxygen and pH 7 (± 1).
- Concentration of sludge: 2979 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation 1

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation 2

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 25.0 °C
- pH: 6.78 - 6.92 (sludge + test substance), 5.92 (water + test substance)
- pH adjusted: yes
- Suspended solids concentration: 30 mg/L

TEST SYSTEM
- Culturing apparatus: test flasks with a fill volume of 300 mL
- Number of culture flasks/concentration: 3
- Measuring equipment: Coulometer Type OM-2001A, Ohkura Electric Co., Ltd

SAMPLING
- Sampling frequency: (BOD) continuous
- Sampling method: (BOD) manometric method equipped with Coulometer

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 1 replicate
- Abiotic sterile control: no
- Toxicity control: no
- Other: Abiotic control: yes, 1 replicate; Activity control: yes, 1 replicate

Reference substance

Reference substance:

aniline

Results and discussion

Test performance:

This test is valid because the differences between the maximum and the minimum in the degradation rates of " sludge + test substance " were less than 20% ( 5% from BOD, 4% from direct determination ( LC ) and 6% from TOC ), and the degradation rate of activity control substance, aniline, calculated from BOD was not less than 60% ( 83% ) after 14 days exposure.

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

1

Sampling time:

28 d

Remarks on result:

other: BOD, mean of three replicates

% Degradation 2

Parameter:

% degradation (test mat. analysis)

Value:

19

Sampling time:

28 d

Remarks on result:

other: LC-analysis, mean of three replicates

% Degradation 3

Parameter:

% degradation (TOC removal)

Value:

11

Sampling time:

28 d

Remarks on result:

other: TOC analysis, mean of three replicates

BOD5 / COD results

Results with reference substance:

83% after 14 d, 60% pass level was reached

Any other information on results incl. tables

Table 1: Degradation rates based on oxygen consumption

			7th day			14th day			21st day			28th day
Test solutions	Amount of test substance added [mg]	TOD [mg]	BOD [mg]	Degradation rate		BOD [mg]	Degradation rate		BOD [mg]	Degradation rate		BOD [mg]	Degradation rate
Sludge + aniline	29.9	89.9	69.6	73		80.4	83		83.0	84		86.3	86
Control blank	-	-	4.2	-		6.1	-		7.6	-		9.1	-
Sludge + test substance	30.0	37.8	4.2	0	3	7.5	4	7	8.7	3	5	8.7	-1	1
Sludge + test substance	30.0	37.8	5.0	2		8.2	6		9.6	5		9.6	1
Sludge + test substance	30.0	37.8	6.5	6		9.7	10		10.5	8		10.5	4
Water + test substance	30.0	-	0.0	-		0.1	-		0.1	-		1.2	-

Table 2: Material balance by direct determination (LC-analysis)

Test solutions	Residual rate of test substance [%]	Production rate of transformation product 1 [%]	Production of transformation product 2 [%]	Material balance [%]
Sludge + test substance	83	9	0	92	94
Sludge + test substance	86	11	0	97
Sludge + test substance	82	10	0	92
Water + test substance	104	0	0	104

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test substance was not readily biodegradable in the test.

Executive summary:

The biodegradation test was conducted in accordance with OECD 301C.

From results of oxygen consumption (BOD) measurement, the degradation rate was 1 % on average.

From results by LC analysis, the residual rate of test item was 84% on average in "sludge + test substance" and 104% in "water + test substance ", and the degradation rate of test item was 19% on average.

From results by IC analysis, the production rate of sulphate ion was 15% on average in "sludge + test substance" and 0%"' in "water + test substance ".

From results by total organic carbon (TOC) measurement, the residual rate was 98% on average in "sludge + test substance " and 110% in " water + test substance ", and the degradation rate was 11 % on average.

It is concluded from these results that the test substance was not readily biodegradable in the test.