α-hydroxy-β,β-dimethyl-γ-butyrolactone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline study, scientifically accepable however no information was given on the purity of the test substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412 L 11.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

α-hydroxy-β,β-dimethyl-γ-butyrolactone

EC Number:

209-963-3

EC Name:

α-hydroxy-β,β-dimethyl-γ-butyrolactone

Cas Number:

599-04-2

Molecular formula:

C6H10O3

IUPAC Name:

(3R)-dihydro-3-hydroxy-4,4-dimethyl-2(3H)-furanone

Details on test material:

- Name of test material (as cited in study report): D-Pantolactone (crystalline)
Purity: no data
Water solubility: 950 g/l

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The stock solution of nominal 2000 mg/l in a culture medium was prepared. Following this, serial dilutions of this solution were prepared in order to obtain the desired test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain:
- Source: Daphnia magna Straus, originally obtained from the Bundesgesundheitsamt, Berlin.
- Age at study initiation (mean and range, SD): 2 - 24 hours
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Feeding during test
- Food type:
- Amount:
- Frequency:


ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food: 1 x daily grown Green algae culture (Scenedesmus subspicatus)
- Feeding frequency:
- Health during acclimation (any mortality observed):


QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

21 °C

pH:

7.2 - 7.7

Dissolved oxygen:

7.9 - 8.2 mg/l

Nominal and measured concentrations:

Nominal test concentrations: 18, 32, 58, 100, 180, 320, 580 mg/l. Additionally, a control was tested in parallel.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker
- Fill volume: 20 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chemistry: according to the test guideline DIN 38412, L 11
- Na /K ration: 20:1
- Ca/mg ratio: 5:1
- Conductivity: 658 µS/cm



OTHER TEST CONDITIONS
- Light intensity: 10 [µE/qm * s]


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24, 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 580 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 580 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 580 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Cumulative immobilization of daphnia after x hours [%]: 
Nominal test concentration (mg/l) 
        /   3 /   6 /  24 /  48  /
Control /   0 /   0 /   0 /   0 /
 18     /   0 /   0 /   0 /  15 /
 32     /   0 /   0 /   0 /  20 /
 58     /   0 /   0 /   0 /   0 /
100     /   5 /   5 /   5 /   5 /
320     /   0 /   0 /   0 /   0 /
580     /   0 /   0 /   0 /   0 /