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EC number: 224-506-8
CAS number: 4390-04-9
Table 1. Dissolved oxygen
concentrations (mg/L) in the closed bottles.
Oxygen concentration (mg/L)
Oco = Seawater
(inoculum) with mineral nutrient solution, with 0.3 mL of silicone oil
but without test material.
Ot = Seawater
(inoculum) with mineral nutrient solution with test material in silicone
oil (1.0 mg/L).
Oc = Seawater
(inoculum) with mineral nutrient solution but without test material and
(inoculum) with mineral nutrient solution with sodium acetate (6.7 mg/L).
Table 2. Oxygen consumption (mg/L)
and the percentages biodegradation (BOD/ThOD) of DEV 2008-016 and sodium
acetate in the closed bottle test inoculated with seawater
Oxygen consumption (mg/L)
In order to assess the biotic degradation in
seawater, a biodegradability test was performed which allows the
biodegradability to be measured in seawater. The biodegradability was
determined in the Closed Bottle test performed according to slightly
modified OECD Test Guidelines, and in compliance with the OECD
principles of Good Laboratory Practice.
DEV 2008 -016 did not cause a reduction in
the endogenous respiration. The test substance is therefore considered
to be non-inhibitory to the inoculum.
DEV 2008 -016 was biodegraded 74% at day 28
in the Closed Bottle test. Hence this substance should be classified as
The test is valid as shown by an endogenous
respiration of 1.0 mg/L and by the total mineralisation of the reference
compound, sodium acetate. Sodium acetate was degraded 85% of its
theoretical oxygen demand after 28 days. Finally, the most important
criterion was met by oxygen concentrations >0.5 mg/L in all bottles
during the test period.
Biodegradation in seawater
Several studies conducted according to OECD
306 guideline were available and all concluded to the ready
biodegradability of C14-20
Aliphatics (≤2% aromatic)i n seawater according to OECD
criteria for biodegradability in seawater.
Biodegradation in sediment
Aliphatics (≤2% aromatic)
are readily biodegradable. Therefore, simulation tests in sediment are
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