Registration Dossier
Registration Dossier
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EC number: 203-615-4 | CAS number: 108-78-1
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Poor description of the part of the publication on the inhibition of soil microorganisms.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Nitrification of Triazine Nitrogen
- Author:
- Hauck RD, Stephenson HF
- Year:
- 1 964
- Bibliographic source:
- Journal of agricultural and food chemistry, 12, 147-151
Materials and methods
- Principles of method if other than guideline:
- Perfusion of test substance solution through soil. Determination of produced NO3- from added ammonium, and possible inhibition by melamine (or cyanuric acid).
- GLP compliance:
- no
Test material
- Reference substance name:
- Melamine
- EC Number:
- 203-615-4
- EC Name:
- Melamine
- Cas Number:
- 108-78-1
- Molecular formula:
- C3H6N6
- IUPAC Name:
- 1,3,5-triazine-2,4,6-triamine
- Details on test material:
- Melamine (N-content: 66.5 %), reagent grade,and also Cyanuric acid (N: 32.4 %) were tested. Melamine was obtained from American Cyanamid Co.
Constituent 1
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 165 d
Test conditions
- Test temperature:
- 25 °C
- Details on test conditions:
- Silty clay loam was used, pH 8.2.
- Nominal and measured concentrations:
- Ca. 490 mg melamine / kg soil (10 mg N of melamine in 30 g soil).
And also: 10mg N of cyanuric acid in 30 g soil.
Perfusion was performed with and without 10 mg N of ammonium.
Results and discussion
- Details on results:
- Melamine (and cyanuric acid) perfusing with added ammonium slightly delayed the nitrification of ammonium by ca. 2 days.
Applicant's summary and conclusion
- Conclusions:
- Melamine only slightly inhibits nitrifying soil microorganisms.
- Executive summary:
Melamine solutions (ca. 490 mg melamine/ kg soil) perfusing through silty clay loam soil slightly delayed the rate of nitrate formation from added ammonium by ca. 2 days.
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