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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Remarks:
Conducted to OECD test guideline. GLP study with chemical analysis by HPLC
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Biphenyl-4,4’-diol / CAS 92-88-6
Analytical monitoring:
yes
Details on sampling:
Samples of prepared test medium were taken at 0 hours (fresh) and 24 hours (old)
Vehicle:
yes
Details on test solutions:
Test solution prepared by dissolution of weighed quantities of test substance into solvent dimethyl formamide (DMF) followed by dilution in Elendt M4 medum at a volume of 100 uL/L
Test organisms (species):
Daphnia magna
Details on test organisms:
Test organisms were supplied from an internal laboratory culture that was fed chlorella vulgaris (0.2 mg Carbon)
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
NA
Hardness:
244 to 248 (0-24 hours)
Test temperature:
19.9 to 20.1°C
pH:
8.3 to 8.5
Dissolved oxygen:
8.5 to 8.8 mg/L
Salinity:
NA
Nominal and measured concentrations:
Nominal concentrations of 0.5, 0.85, 1.4, 2.4, 4.1 and 7.0 mg/L
Measured concentrations: 0.513, 0.86, 1.44, 2.48, 4.17 and 7.25 mg/L
Details on test conditions:
A 48 hour acute toxicity test was conducted in accordance with OECD 202 Acute toxicity to Daphnia magna, under semi-static conditions. Based on the results of a range finding test a definitive test was conducted at nominal concentration of 0.5, 0.85, 1.4, 2.4, 4.1 and 7.0 mg/L. Four
replicate test vessels were prepared per test concentration containing 100 mL of test medium and 5 Daphnia per vessel (20 per concentration in
total).

The test was conducted with a 16 hour light and 8 hour dark lighting regime at (ca. 800 Lux).

Test solutions were prepared by direct weighing and dilution in a solvent vehicle (DMF) prior to dilution (100 µL/L) in Elendt M4 medium.

Concentrations were confirmed at 0 and 24 hours, 103 to 106% and 99 to 102% respectively (LOD 0.0002 mg/L).
Reference substance (positive control):
not specified
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 7.25 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.86 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
LC100
Effect conc.:
> 7.25 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.76 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: between 1.47 and 2.11
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.513 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
7.25 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
There was no control or solvent control immobility during the conduct of the test
Results with reference substance (positive control):
NA
Reported statistics and error estimates:
Probit

Immobility results

Nominal concentration (mg/L)

Geometric mean measured concentration
(mg/L)

% Immobility

24 hours

48 hours

Control

-

0

0

Solvent control

-

0

0

0.5

0.513

0

0

0.85

0.860

0

5

1.4

1.44

10

50

2.4

2.48

10

65

4.1

4.17

20

95

7.0

7.25

25

100

Analytical results

Nominal concentration (mg/L) Measured concentrations (mg/L) Geometric mean measured concentrations (mg/L)
0 hours 24 hours
Control <LOD <LOD <LOD
Solvent control <LOD <LOD <LOD
0.5 0.52 0.506 0.513
0.85 0.879 0.842 0.86
1.4 1.47 1.42 1.44
2.4 2.54 2.43 2.48
4.1 4.21 4.14 4.17
7 7.35 7.15 7.25
Validity criteria fulfilled:
yes
Conclusions:
The 48 hour EC50 was calculated to be 1.76 mg/L
The 48 hour NOEC was considered to be 0.860 mg/L
Executive summary:

A GLP 48 hour semi-static acute toxicity test was conducted with 4,4 -biphenol in accordance with OECD 202 Acute toxicity to Daphnia magna, under semi-static conditions using Elendt M4. Based on the results of a range finding test a definitive test was conducted at nominal concentration of 0.5, 0.85, 1.4, 2.4, 4.1 and 7.0 mg/L. 

Geometric mean measured concentrations were: 0.513, 0.86, 1.44, 2.48, 4.17 and 7.25 mg/L.

The 48 hour EC50was calculated to be 1.76 mg/L (based on geometric mean measured concentrations)

The 48 hour NOEC was considered to be 0.860 mg/L (based on geometric mean measured concentrations)

Description of key information

Nominal concentrations: 3.0, 5.3, 9.5, 17 and 30 mg/L (spacing factor 1.8)
Measured concentrations: 101-105% (0 hours), 99-106% (24 hours) analysis by HPLC.
96 hour EC50 = 1.76 mg/L (CI 95% 1.47 to 2.11 mg/L)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
1.76 mg/L

Additional information

A GLP 48 hour semi-static acute toxicity test was conducted with biphenyl-4,4'-diol in accordance with OECD 202 Acute toxicity to Daphnia magna, under semi-static conditions using Elendt M4. Based on the results of a range finding test a definitive test was conducted at nominal concentration of 0.5, 0.85, 1.4, 2.4, 4.1 and 7.0 mg/L. 

Geometric mean measured concentrations were: 0.513, 0.86, 1.44, 2.48, 4.17 and 7.25 mg/L.

The 48 hour EC50was calculated to be 1.76 mg/L (based on geometric mean measured concentrations)

The 48 hour NOEC was considered to be 0.860 mg/L (based on geometric mean measured concentrations)