Aniline

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Principles of method if other than guideline:

The test substance is applied orally by gavage in graduated doses to seven groups of cats, one dose being used per group.
Subsequently, observations of deathss and clinical effects including the determination of methemoglobin level in the blood are made.

GLP compliance:

no

Test type:

other: acute test

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Aniline

EC Number:

200-539-3

EC Name:

Aniline

Cas Number:

62-53-3

Molecular formula:

C6H7N

IUPAC Name:

aniline

Details on test material:

- Name of test material (as cited in study report): aniline
- Analytical purity: p. a.

Test animals

Species:

cat

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: BASF AG, Department of Toxicology
- Weight at study initiation: 2.4 kg (male), 1.9 kg (female)
- Diet (e.g. ad libitum): A soft feed made of cooked oat flakes, Latz-Purin and sodium chloride; sometimes the meat of whales; ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: aqueous emulsion in traganth

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 0.05 - 1% aqueous emulsion in traganth

Doses:

1.0, 2.6, 5.1, 10.2, 25.6, 51.1 and 102.2 mg/kg

No. of animals per sex per dose:

2 animals per dose (two males, two females or one male and one female); in total 14 animals

Control animals:

no

Details on study design:

- Duration of observation period following administration: 3 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, methaemoglobin formation

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

approximate LD50

Effect level:

>= 102 mg/kg bw

Any other information on results incl. tables

One out of two male cats died after administration of 102.2 mg/kg bw aniline. Light cyanosis was seen at day 1 after application of 2.6 mg/kg. Doses of 5.1, 10.2 and 25.6 mg/kg bw caused cyanosis in all animals. 51.1 mg/kg caused severe cyanosis with staggering gait, atony and vocalisation. In the high dose animals (102.2 mg/kg bw), severe cyanosis accompanied by accelerated breath and lateral position.

	Animal	Haemoglobin [%] Hours after administration
Dose [mg/kg]		Pre-test	2	4	6	24	30	48	72
102.2	1	0.5	74.1	71.2	79.9	28.2*	-	-	-
102.2	2	0.2	73.8	77.4	78.8	70.5	56.9	4.8	3.1
51.1	3	0	66.1	76.0	73.0	2.5	2.0	0	0
51.1	4	3.6	55.6	63.2	35.1	2.0	1.2	0.4	0.4
25.6	5	0	60.6	71.3	70.6	1.8	1.2	0.9	0
25.6	6	0	62.9	65.1	61.1	2.4	1.0	1.1	0
10.2	7	0	66.8	64.0	49.7	0.5	0.5	0.5	1.2
10.2	8	0	41.8	34.5	20.2	0.9	0.9	0.3	1.2
5.1	9	0	29.0	12.8	4.5	1.0	0.9	0	0.8
5.1	10	0	24.6	8.1	0	0.6	0	0	0
2.6	11	0	8.1	2.6	0.3	0	1.3	0.7	0.7
2.6	12	0.8	10.2	3.4	0	0.6	0	0	0
1.0	13	0.5	3.4	2.6	1.2	0.8	0	1.0	0
1.0	14	0.5	6.0	1.6	1.0	0.5	0	0	0

* Blood was taken after death.