Butan-1-ol

Toxicity to reproduction: other studies

Administrative data

Endpoint:

toxicity to reproduction: other studies

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

unsuitable test system

Remarks:

No validated or appropriate exposure route, especially not for an irritant. Questionable relevance as valid in vivo studies are available

Data source

Reference

Reference Type:

publication

Title:

Effects of Alcohols on murine preimplantation development:relationship to relative membrane disordering potency

Author:

Kowalczyk et al

Year:

1996

Bibliographic source:

Alcoholism:Clinical and Experimental Research, Vol.20, No.3

Materials and methods

Principles of method if other than guideline:

Murine preimplantation embryos were exposed to n-butanol (0.05 to 1%) and development of the embryo as well as intracellular calcium levels were observed.

GLP compliance:

not specified

Type of method:

in vitro

Test animals

Species:

mouse

Strain:

other: in vitro culture of murine preimplantation embryos

Details on test animals or test system and environmental conditions:

Female mice (strain NSA, colony 202) were superovulated and paired with B6SJL/J males for mating. Female mice were sacrificed on gestation day 2 for collection of two-cell embryos or on gestation day 3 for collection of eight-cell morulae.

Administration / exposure

Details on exposure:

Embryos at the two-cell stage were washed and exposed to 0, 0.05, 0.1, 0.2 or 1 % n-butanol (7 to 135mM) for 24h in medium. After incubation, embryos were washed and cultured for 5 days to observe their development to the blastocyst stage. In addition, 8-cell mouse morulae were exposed tp 0.1 or 1% n-butanol and intracellular calcium concentrations were measured fluorecently.

Doses / concentrations

Remarks:

conc. tested: 0., 0.01, 0.05, 0.1 0.2 or 1% (w/v) n-butanol

Results and discussion

Effect levels

Remarks on result:

not measured/tested

Observed effects

Exposure to n-butanol decreased the percentage of embryos reaching the blastocyst stage where the maximum reduction occured at the lowest concentration. In addition, 1% n-butanol increased the intracellular calcium levels in an 8-cell mouse morulae.