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Ecotoxicological information

Toxicity to terrestrial plants

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Reference
Endpoint:
toxicity to terrestrial plants
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March 2016 - August 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Information is reliable for this endpoint, the method is a guideline study and the information is needed for REACH. Therefore the information is adequate
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Iso E Super
- Pale yellow liquid
- Batch no SM15068074
-Expiry data: 4th June 2017
Analytical monitoring:
yes
Remarks:
Test substance has been confirmed in the stock solution
Details on sampling:
Duplicat specimen of the homogenous stock solutions and the two spicemen of the matrix (acetone) as control were taken.
Vehicle:
yes
Remarks:
Aceton
Details on preparation and application of test substrate:
The test substance was dissolved in aceton and mixed with fine quartz sand before the test substance treated sand was mixed into the soil
Species:
Allium cepa
Species:
Avena sativa
Species:
Cucumis sativus
Species:
other: Solanum lycopersicum
Species:
Glycine max (G. soja)
Species:
Brassica napus
Test type:
seedling emergence and seedling growth test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
21 d
Remarks:
Fourteen to 21 days after 50% germination of the control
Post exposure observation period:
No
Justification for exposure duration:
Exposure duration is as described in the guidelines
Test temperature:
21.5
pH:
5.9
Moisture:
71
Nominal and measured concentrations:
Nominal concentration were used
Reference substance (positive control):
no
Key result
Species:
Brassica napus
Duration:
14 d
Dose descriptor:
EC10
Effect conc.:
30 mg/kg soil dw
Nominal / measured:
nominal
Basis for effect:
growth
Key result
Species:
Glycine max (G. soja)
Duration:
14 d
Dose descriptor:
EC10
Effect conc.:
24 mg/kg soil dw
Nominal / measured:
nominal
Basis for effect:
growth
Key result
Species:
other: Solanum lycopersicum
Duration:
14 d
Dose descriptor:
EC10
Effect conc.:
15 mg/kg soil dw
Nominal / measured:
nominal
Basis for effect:
growth
Key result
Species:
Cucumis sativus
Duration:
14 d
Dose descriptor:
EC10
Effect conc.:
50.3 mg/kg soil dw
Nominal / measured:
nominal
Basis for effect:
growth
Key result
Species:
Avena sativa
Duration:
14 d
Dose descriptor:
EC10
Effect conc.:
19.4 mg/kg soil dw
Nominal / measured:
nominal
Basis for effect:
growth
Key result
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
24 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Reported statistics and error estimates:
Statistical analysis of the control plants and solvent control plants:
Fresh weight data were tested for normal distribution and homogeneity of variance (F test,  = 0.05). If the data were normally distributed and homogeneous the Student t-test (pairwise comparison, two-sided,  = 0.05) was used. If the data were not normally distributed the Mann-Whitney U-test (pairwise comparison, two-sided,  = 0.05) was used.

 

 

Validity criteria fulfilled:
yes
Remarks:
Germination Rate of the Control Seeds wat at least 70%; Mean Survival of Emerged Control Seedlings was > 90%; the control seedlings did not exhibit phytotoxic effects.
Conclusions:
The EC10 of 27 mg/kg soil dw will be used for the CSA, which is an average EC10 over 6 plant species.
Executive summary:

In a terrestrial plant test according to OECD TG 208 under GLP, 6 plant species were tested for seedling emergence, growth and phytotoxic effects (chlorosis, necrosis and abnormal growth). The overall EC10 on leaf fresh weight being the most sensitive parameter resulted in 27 mg/kg soil dw. The doses were selected based on a dose range finding study and were 12.3, 37, 111, 333 and 1000 mg/kg soil dw. The concentration in the stock solution was verified but actual measures in the soil were not performed. The soil is a sandy loam and was sterilised before use. All particles were < 0.2cm. The organic matter content is 0.67 +/-0.03%. The following dicotyls were used: Brassica napus, Glycine max, Solanum lycopersicum and Cucumis sativus. The following monocotyles were use: Avena sativa and Allium cepa. Untreated seeds were sewn in pots and plants were grown for 14 days, except Allium cepa which was grown for 21 days. Fresh weight of the leaves of plants was the more sensitive endpoint compared to germination, phytotoxicity and mortality. The EC10 of all six plant species was averaged as the number of species =>5.

Description of key information

See below.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for terrestrial plants:
27 mg/kg soil dw

Additional information

In a terrestrial plant test according to OECD TG 208 under GLP, 6 plant species were tested for seedling emergence, growth and phytotoxic effects (chlorosis, necrosis and abnormal growth). The overall EC10 on leaf fresh weight being the most sensitive parameter resulted in 27 mg/kg soil dw. The doses were selected based on a dose range finding study and were 12.3, 37, 111, 333 and 1000 mg/kg soil dw. The concentration in the stock solution was verified but actual measures in the soil were not performed. The soil is a sandy loam and was sterilised before use. All particles were < 0.2cm. The organic matter content is 0.67 +/-0.03%. The following dicotyls were used: Brassica napus, Glycine max, Solanum lycopersicum and Cucumis sativus. The following monocotyles were use: Avena sativa and Allium cepa. Untreated seeds were sewn in pots and plants were grown for 14 days, except Allium cepa which was grown for 21 days. Fresh weight of the leaves of plants was the more sensitive endpoint compared to germination, phytotoxicity and mortality. The EC10 of all six plant species was averaged as the number of species =>5.