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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
05/01/1995-17/02/1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to OECD TG 301F in compliance with GLP, without devations that influence the quality of the results.
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not relevant
Analytical monitoring:
not specified
Details on sampling:
See 5.2.1 Rudio_95 - E03_RIFM 33492_1995_Manometric Respirometry Test
Vehicle:
no
Details on test solutions:
See 5.2.1 Rudio_95 - E03_RIFM 33492_1995_Manometric Respirometry Test
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
See 5.2.1 Rudio_95 - E03_RIFM 33492_1995_Manometric Respirometry Test
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
42 d
Test temperature:
See 5.2.1 Rudio_95 - E03_RIFM 33492_1995_Manometric Respirometry Test
pH:
See 5.2.1 Rudio_95 - E03_RIFM 33492_1995_Manometric Respirometry Test
Dissolved oxygen:
See 5.2.1 Rudio_95 - E03_RIFM 33492_1995_Manometric Respirometry Test
Nominal and measured concentrations:
See 5.2.1 Rudio_95 - E03_RIFM 33492_1995_Manometric Respirometry Test
Details on test conditions:
See 5.2.1 Rudio_95 - E03_RIFM 33492_1995_Manometric Respirometry Test
Reference substance (positive control):
no
Key result
Duration:
42 d
Dose descriptor:
NOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
The biodegradation curves obtained with Aniline alone and with OTNE + Aniline show no toxic effect of OTNE on the micro-organisms at the test concentration. See attached background material in 5.2.1 Rudio_95 - E03_RIFM 33492_1995_Manometric Respirometry Test for biodegradation curves.
Validity criteria fulfilled:
not applicable
Conclusions:
At the concentration used in the test (100 mg/l), OTNE is not inhibitory to the micro-organisms.
Executive summary:

The toxicity of OTNE was determined as part of a Manometric Respiratory Test according to the OECD Guidelines for Testing of Chemicals, Method No. 301 F. The biodegradation of the reference substance Aniline was tested, with and without addition of 100 mg/l OTNE. This test showed no toxic effect of OTNE on the micro-organisms at the tested concentration, therefore the NOEC was at or above 100 mg/l.

Description of key information

The toxicity of OTNE was determined as part of a Manometric Respiratory Test according to the OECD Guidelines for Testing of Chemicals, Method No. 301 F. The biodegradation of the reference substance Aniline was tested, with and without addition of 100 mg/l OTNE. This test showed no toxic effect of OTNE on the micro-organisms at the tested concentration, therefore the NOEC was at or above 100 mg/l.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information