Tetrasodium 4,4'-bis[[4-[bis(2-hydroxypropyl)amino]-6-[(4-sulphonatophenyl)amino]-1,3,5-triazin-2-yl]amino]-stilbene-2,2'-disulphonate

Short-term toxicity to aquatic invertebrates

Description of key information

EC50 48h Daphnia magna > 113 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

113 mg/L

Additional information

The short-term toxicty to aquatic invertebrates was evaluated in a weight-of-evidence approach by considering data on the substance and on the similar substance 3a-MSA. Justitification for Read Across is given in Section 13 of IUCLID.

Two tests on the substance 4 -MSA were performed: a well described study on Daphnia Magna reported no effect at 1000 mg/l, but exposure is limited at 24 hours (Ellgehausen, 1986). A second study was performed at 48 hours, but the highest dose tested was 100 mg/l, no concentration analytical monitoring was performed and non-dissolved particles were separated from the solution by filtration, which could result in major differences between nominal and actual concentrations (Bayer AF, 1999).

Both results are consistent and in agreement with the expected results for the category of Stilbene Fluorescent Whitening Agents and they support the endpoint in a weight of evidence approach, together with the results of the most similar substance within the category 3a-MSA.

This substance can be considered the best representative within the category, since it has the same sulphonation degree as 4 -MSA, a very high structural similarity (Tanimoto distance > 0.9) and a very high water solubility (650 g/l vs 1000 g/l of 4 -MSA) (details in the category Justification Report, attached to the Section 13 of the dossier).

For the chemical safety assessment the results obtained by the study on the similar substance 3a-MSA conducted according to the EU method C2 and under GLP conditions (EC50 > 113 mg/l)