Registration Dossier
Registration Dossier
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EC number: 200-589-6 | CAS number: 64-67-5
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Data are lacking in the publication but the study has been performed by the team that developped the test. So it is considered that it has been appropriately conducted. However, as DES is corrosive, and LLNA is known to induce false positive response for very irritant substances, the validity of this positive response is doubtful.
Data source
Reference
- Reference Type:
- publication
- Title:
- Structure activity relationships in skin sensitization using the murine local lymph node assay
- Author:
- Ashby J, Basketter DA, Paton D, Kimber I
- Year:
- 1 995
- Bibliographic source:
- Toxicology 103: 177-194
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Diethyl sulphate
- EC Number:
- 200-589-6
- EC Name:
- Diethyl sulphate
- Cas Number:
- 64-67-5
- Molecular formula:
- C4H10O4S
- IUPAC Name:
- diethyl sulfate
- Details on test material:
- no further data
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- not specified
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 1; 2.5; 10 %
- No. of animals per dose:
- no data
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: 0.8 - 1.9 and 12.0 for 1; 2.5 and 10% respectively
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: no data
Any other information on results incl. tables
no further data
Applicant's summary and conclusion
- Interpretation of results:
- sensitising
- Remarks:
- Migrated information
- Conclusions:
- DES is positive in this LLNA test
- Executive summary:
A LLNA test has been perfomed with DES as one of the 106 chemicals tested. A SAR was developed based on the electrophilicity of the substance.
DES was considered as a sensitizer in this test.
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