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Short-term toxicity to aquatic invertebrates

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Description of key information

48-hour EL*50 = 43 mg/L loading rate WAF; 48-hour NOEC =  32 mg/L loading rate WAF, OECD 202, EU Method C.2, Priestly & Mullee (2010)

Key value for chemical safety assessment

Additional information

A study was performed to assess the acute toxicity of the test material to Daphnia magna. The method followed that described in the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp, Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.

Following a preliminary range-finding test, twenty daphnids (2 replicates of 5 animals) were exposed to Water Accommodated Fractions (WAFs) of the test material over a range of nominal loading rates of 10, 18, 32, 56 and 100 mg/L for 48 hours under at a temperature of 21°C under static test conditions. The number of immobilised Daphnia were recorded after 24 and 48 hours.

A positive control conducted approximately every six months used potassium dichromate as the reference item. Daphnia magna were exposed to an aqueous solution of the reference item at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L for 48 hours at a temperature of approximately 20°C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours.

Under the conditions of the test, the48-hour EL*50 for the test material, based on nominal loading rates, was 43 mg/L loading rate WAF with 95% confidence limits of 41 - 46 mg/L loading rate WAF. The No Observed Effect Loading rate was 32 mg/L loading rate WAF.

The 48-hour EC50 for the reference item to Daphnia magna based on nominal concentrations was 0.78 mg/L with 95% confidence limits of 0.58 – 0.72 mg/L. The No Observed Effect Concentration was 0.32 mg/L.