Benzoic acid, C12-15-alkyl esters

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16. Feb. 1979 - 27. Apr. 1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

no

Test type:

other: trade name

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzoic acid, C12-15-alkyl esters

EC Number:

270-112-4

EC Name:

Benzoic acid, C12-15-alkyl esters

Cas Number:

68411-27-8

Molecular formula:

C19-22 H30-36 O2

IUPAC Name:

Benzoic acid, C12-15-alkyl esters

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Summit View Farm, Belvidere, New Jersey
- Weight at study initiation: 200 -246 g
- Diet: Wayne animal feed, ad libitum
- Water: ad libitum
- Acclimation: animals were conditioned prior to use

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: plastic chamber
- Exposure chamber volume: three-cubic-foot
- Source and rate of air: preconditioned supportive air at 5 L/minute
- System of generating particulates/aerosols: Preconditioned supportive air was supplied and the test material was presented from a compressor activated generator or timer operated solenoid to continue a nominal chamber concentration of 200 mg / liter air

TEST ATMOSPHERE
- nominal concentration of 200 mg / liter air

VEHICLE
- Composition of vehicle (if applicable): air
- Concentration of test material in vehicle (if applicable): 200 mg / liter air

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

1 h

Concentrations:

200 mg / liter air

No. of animals per sex per dose:

5 males, 5 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations daily (1, 3, 6, 24 hours after exposure on day 1) and weighing at start and end of the experiment
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight , necropsy

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 200 mg/L air

Based on:

test mat.

Exp. duration:

1 h

Mortality:

Dose Level (mg/l) Sex # dead /# dosed Mortality ( %)
200 5 M : 5 F 1/5 : 0/5 10

Clinical signs:

other: Slight depression observed 1 hour after exposure at all 10 animals, otherwise no abnormal clinical signs within 14 days after exposure.

Body weight:

Body weight gain at 9 survived animals 14 days after exposure.
Body weight loss at 1 animal that died on 9th day after exposure.

Gross pathology:

Animal #1: All lobes of right lung enlarges and consolidated. Animal died on day 9.
Animals #2 - #10: No gross changes observed.

Any other information on results incl. tables

 

Dose (mg/l

Animal No. and Sex

Body weight (g)

Hours:   1    3    6   24

Days:   2   3    4   5   6   7 --14

Body weight (g)

200

1 M

202

SD

N

N

N

N

N

N

N

N

N+  -

156

200

2 M

230

SD

N

N

N

N

N

N

N

N

N    N

296

200

3 M

232

SD

N

N

N

N

N

N

N

N

N    N

324

200

4 M

230

SD

N

N

N

N

N

N

N

N

N    N

290

200

5 M

246

SD

N

N

N

N

N

N

N

N

N    N

312

200

6 F

204

SD

N

N

N

N

N

N

N

N

N    N

222

200

7 F

200

SD

N

N

N

N

N

N

N

N

N    N

226

200

8 F

200

SD

N

N

N

N

N

N

N

N

N    N

262

200

9 F

200

SD

N

N

N

N

N

N

N

N

N    N

210

200

10 F

200

SD

N

N

N

N

N

N

N

N

N    N

220

 

N  = Normal

SD = Slight Depression

+ = Animal Death 

Animal No. 1: All lobes of right lung enlarges and consolidated; died on day 9.

Animal No. 2-10: No gross changes observed.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In the Acute Inhalation Toxicity study on Wistar-derived rats the LC50 of the test material was determined as being > 200 mg/l.
There was a 10% mortality observed in the exposed rats. Under the conditions if this study the test material is considered to be not toxic by inhalation to rats.

Executive summary:

In an Acute Inhalation Toxicity study ten albino rats (5M:5F, 200 -246 g body weight) were exposed to concentrations of 200 mg test material / liter air for 1 hour and observed for two weeks. Mortality, clinical signs and body weight development were monitored during the study. All animals were subjected to gross necropsy.  

Mortality: 10 % (1/10 animals)

Clinical Observations: Slight depression was observed at all animals 1 h after exposure; otherwise no abnormal clinical signs within 14 days observation period.

Body weight: Body weight gain at 9 animals after the 14 days observation period, body weight loss at 1 animal that died after exposure.

Necropsy: No gross changes observed at 9 animals. In the one dead animal all lobes of right lung enlarges and consolidated.

Conclusion: The LC50 of the test material was determined as being > 200 mg/l. Under the conditions if this study the test material is considered to be not toxic by inhalation.