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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

The lowest effect concentration was observed for fully saturated TEA-Esterquat.
The effect concentrations after 30 minutes according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest) are as follows:
EC0: 24.0 mg a.i./L
EC10: 29.6 mg a.i./L
EC50: 60 mg a.i./L

Key value for chemical safety assessment

EC50 for microorganisms:
60 mg/L
EC10 or NOEC for microorganisms:
29.6 mg/L

Additional information

For fully saturated TEA-Esterquat the acute bacterial toxicity was determined according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest). The calculated effect concentrations after 30 minutes were as follows: EC0: 24.0 mg a.i./l, EC10: 29.6 mg a.i./L, EC50: 60 mg a.i./L.

In a study with test substance partially unsaturated TEA-Esterquat the 30 minutes acute bacterial toxicity was determined according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest). The calculated effect concentrations were as follows: 30 min EC0: 30 mg test mat./L, 30 min EC10: 35 mg test mat./L, and 30 min EC50 110 mg test mat./L.

Another study with partially unsaturated TEA-Esterquat did not severely inhibit the respiration rate of activated sludge obtained from a biological treatment plant. The 3h EC50 is > 250 mg test mat./L (nominal).

One further study is available forpartially unsaturatedTEA-Esterquat, but this study was not used for this assessment, because the stock solution of the test item was heated to 50°C. A significant amount of hydrolysis might have taken place.

The above findings are in line with the following results for partially unsaturated TEA-Esterquat.In a 3 hour toxicity study conducted according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test) and EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test), the cultures of activated sludge of a predominantly domestic sewage treatment plant were exposed to partially unsaturated TEA-Esterquat at nominal concentrations of 0, 1, 3, 9, 27, 81, 243 mg/L under static conditions. The 3 h EC50 value based on inhibition of respiration was > 243 mg test mat./L.

No studies on toxicity to microorganisms are available for oleic acid-based TEA-Esterquat. The lowest effect concentration was observed for fully saturated TEA-Esterquat. Therefore, the results of fully saturated TEA-Esterquat are used for the assessment.