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Short-term toxicity to fish

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Description of key information

The lowest effect concentration was observed in a study conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test) under semi-static conditions the LC50 (96 h) of TEA-Esterquat partially unsaturated was determined to be 1.91 mg/L for Oncorhynchhus mykiss.

Key value for chemical safety assessment

LC50 for freshwater fish:
1.91 mg/L

Additional information

In a 96-h acute toxicity study conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test), rainbow trouts (Oncorhynchus mykiss) were exposed to partially unsaturated TEA-Esterquat at nominal concentrations of 0 (control), 1.0, 1.6, 2.56, 4.1, 6.55, 10.55 mg/l under semi static conditions. The mean measured concentrations were determined to be 59 % of nominal concentrations. The 96-h LC50 was 1.91 mg/L (based on mean measured concentrations). Sublethal effects such as difficulties with maintenance of balance and unusual behaviour (reduced activity and or orientation to bottom or surface of the test vessels) were observed within the first three hours in the highest test concentration.

The above findings are in line with the results of 3 further reliable studies for partially unsaturated TEA-Esterquat. In a study performed according to OECD Guideline 203 (Fish, Acute Toxicity Test) under semi-static conditions the LC50 (96 h) of partially unsaturated TEA-Esterquat was determined to be 2.1 mg/L for Danio rerio. The effect concentration (96h-LC50 = 2.6 mg/l)

was corrected because the analytical recovery (HPLC) of test substance was below 80 % of nominal concentration.

Partially unsaturated TEA-Esterquat was classified as toxic toDanio rerioby the study authors. However, according to Directive 67/548/EWG and CLP (EU-GHS), Regulation (EC) No 1272/2008 the criteria for acute aquatic toxicity of fish (96h LC50) is </= 1 mg/L.

There are three further reliable studies available for partially unsaturated TEA-Esterquat performed according to OECD Guideline 203 (Fish, Acute Toxicity Test) under semi-static conditions, but without analytical control. The LC50 (96 h) effect concentrations were 7.48 mg/L (test mat., nominal, based on a.i. > 80 % the 96h LC50 was > 5.98 mg a.i./L) for Danio rerio and 11.23 mg a.i./Land 14.45 mg a.i./L forOncorhynchus mykiss each, respectively. The nominal effect concentrations were corrected for the content of active ingredient.

One additional study is available for Leuciscus idus (Golden orfe). In this study the 24 to 96 h LC 50 of TEA-Esterquat partially unsaturated was 5.1 mg test mat./l (nominal).

These findings are in accordance with a test for oleic acid-based TEA-Esterquat conducted under semi-static conditions resulting in a nominal LC 50 (96 h) of 4.8 mg/L test mat. Analytical control was not performed.

In a flow through test according to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study, 1984)the 96 h and 14 d LC 50 of partially unsaturated TEA-Esterquat were 4.9 mg/L each (nominal) for Danio rerio.No analytical control was performed during the test.