Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acrylate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-07-20 - 2012-09-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

OECD 203, GLP, analytical monitoring.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Analytical evaluation of the concentrations of Isobornyl acrylate and the control were carried out via GC-FID from freshly prepared media after 0 and 72 h, and from 24 h old test media after 24 and 96 h.

Vehicle:

no

Details on test solutions:

Dilution water: purified drinking water of local origin

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

All fish used in the test were gained at Dr.Noack-Laboratorien, from a single brood stock (supplier: Umweltbundesamt, Schichauweg 58, D-12307 Berlin).

Holding:
Fish were held at the test facility at 23  2 °C and diffuse light (0.1-10 µmol photons • m-2 • s-1, natural photoperiod).
The water was changed at least once per week.
The dissolved oxygen concentration was more than 80 % of the air saturation value.

Zebrafish with at least 12 days of acclimatisation and mortality < 5 % within this period of time were used in the test. No disease treatments were administered throughout holding and testing.

Food (Sera Vipan; Sera GmbH, D-52518 Heinsberg) was provided 3 times per week. The amount of food was 4 % of the fish body weight per feeding day.
The test fish were not fed 24 h before the test started.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

52 mg CaCO3/L

Test temperature:

21.7 ± 0.46 °C

pH:

varied between 7.0 and 7.5

Dissolved oxygen:

in all test vessels between 85 % and 100%

Nominal and measured concentrations:

see table below.

Details on test conditions:

Dilution water was prepared according to the test guideline (OECD 203) following internal SOP’s.

Test concentrations:
Aliquots of the stock solution were shaken for 24 h, then they were diluted with purified drinking water (dilution water).


Test priciples and procedure:

10 fish were used for each test concentration including controls.
Only fish in good health and free from any apparent malformation were used.


15-l glass aquaria were used as test vessels and filled with 10 l of test solution. The test animals were added to the previously prepared test solution; controls were kept in dilution water (blank).

The aquaria were slightly aerated via a glass capillary throughout the test.


The fish were exposed to various concentrations of the test substance for a period of 96 hours under semi static conditions. The test medium was changed completely every day. Visible abnormalities observed during the first 6 hours of the experiment were recorded. Mortalities and visual abnormalities were recorded at 24 h, 48 h, 72 h, and 96 h.

The test temperature was 21.8° C to 22.1 ° C. The pH of the dilution water was 7.8 to 8.1. The oxygen saturation in all test vessels was between 86 % and 100 %.
The light/dark rhythm was 14h/10h.

The pH, temperature and the oxygen concentrations of the control and of all the test concentrations were measured daily.

Reference substance (positive control):

not required

Remarks:

No reference item is recommended for this test according to the guidelines.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.704 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.431 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1.15 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

see tables OBSERVATIONS and CUMULATIVE MORTALITY (below)

Reported statistics and error estimates:

Equation: 0 to 100, sigmoidal dose-response, variable slope Y=100/(1+10^((logEC50-X)*Hill Slope))

Sublethal observations / clinical signs:

Concentrations of Isobornyl acrylate

 

Sampling date	2012-09-24 fresh media; 0 h		2012-09-25 old media; 24 h		2012-09-27 fresh media; 72 h		2012-09-28 old media; 96 h
Start of analysis	2012-09-24		2012-09-25		2012-09-27		2012-09-28
	Isobornyl acrylate
Nominal test item conc. [mg/L]	Meas. conc. [mg/L]	RR [%]	Meas. conc. [mg/L]	RR [%]	Meas. conc. [mg/L]	RR [%]	Meas. conc. [mg/L]	RR [%]	Geometric mean measured conc. [mg/L]
8.0	6.84^1)	86	5.76	72	*		*		6.28
3.64	3.03^1)	83	2.28	63	*		*		2.63
1.65	1.20^1)	72	0.895	54	1.66	101	0.990	60	1.15
0.751	0.361^1)	48	0.298	40	0.728	97	0.439	58	0.431
0.341	0.120^1)	35	0.101	30	0.312	91	0.169	50	0.159
0.155	0.0737^1)	48	0.0628	41	0.0917^2)	59	0.0666	43	0.0732
Control	< LOQM		< LOQM		< LOQM		< LOQM		< LOQM

        conc.  = concentration

        Meas. conc. = Measured concentration of the test item (dilution factor taken into account)

        RR  = Recovery rate related to the nominal concentration of the test item

        LOQ_M = Limit of quantification of theanalytical method(0.01 mg/L of the test item)

        *   = no samples due to 100 % Mortality

        1)  = Reanalysed 25.09.2012, mean value of 3 Injections (dilution factor taken into account).

        2)  = Reanalysed 28.09.2012, mean value of 3 Injections (dilution factor taken into account).

OBSERVATIONS

 

Geometric mean test item concentration	Effect *	Number of fish affected at observation time [hours]
[mg/L]		24	48	72	96
6.28	(E)	7/7	---	---	---
2.63	(E)	3/7	4/4	---	---
	(2.3)	4/7	-	---	---
1.15	(E)	-	-	5/7	2/2
	(2.3)	-	-	2/7	-
	(1)	7/7	7/7	-	-
0.431	(1)	7/7	7/7	7/7	7/7
0.159	(1)	7/7	7/7	7/7	7/7
0.073	(1)	7/7	7/7	7/7	7/7
Control	(1)	7/7	7/7	7/7	7/7

              *) The numbers in brackets correspond to the following observations: 

 

                         (1)      =behaviour                                           

                         (2.3)   = Staggering                                                      (E)    = Exitus letalis

                          -   = No observations                                             ---     = No observation due to 100 % mortality

CUMULATIVE MORTALITY

Geometric mean  test item concentration	Cumulative mortality [%] at observation time [hours]
[mg/L]	24	48	72	96
6.28	100	100	100	100
2.63	43	100	100	100
1.15	0	0	71	100
0.431	0	0	0	0
0.159	0	0	0	0
0.073	0	0	0	0
Control	0	0	0	0

 

Validity criteria fulfilled:

yes

Conclusions:

In a valid guideline study (OECD 203, semi-static conditions), the 96 hr LC50 for Danio rerio (Teleostei, Cyprinidae) is 0.704 mg/L .

Executive summary:

 In a valid 96-hr acute toxicity study according to OECD TG 203 with GLP, Danio rerio were exposed to Isobornyl acrylate at 0 (control), 0.155 – 0.341 – 0.751 – 1.65 – 3.64 – 8.00 mg/L nominal and 0 (control), 0.073 – 0.159 – 0.431 – 1.15 – 2.63 – 6.28 mg/L measured concentrations under semi-static conditions.

The 96-h LC₅₀was 0.704 (0.431 – 1.15 95 % confidence limits) mg a.i./L based on mortality.

The LC₁₀₀and NOEC values, based on mortality effects, were 1.15 mg/L and 0.431 mg a.i./L, respectively. The effect concentrations refer to the geometric mean measured concentrations.

 

Results Synopsis

LC₅₀: 0.704 mg a.i./L                         95% C.I.: 0.431 - 1.15 mg a.i./L

NOEL: 0.431 mg a.i./L                      

LC₁₀₀:  1.15 mg a.i./L}                      

Endpoint(s) Effected: mortality

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

One reliable study for Isobornyl acrylate on freshwater fish acc. OECD 203 is available. LC50 (96h) on Danio rerio was determined to be 0.704 mg/L 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

0.704 mg/L