Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2008-05-15 to 2008-06-12
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: 2b Guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Version / remarks:
It is not clearly specified which version was used.
Qualifier:
according to guideline
Guideline:
other: SS-EN ISO 7827:1996
GLP compliance:
no
Oxygen conditions:
aerobic
Remarks:
To maintain aerobic conditions in the mediums air was bubbled from an air pump through a long glass tube which ended at the bottom of the flask.
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
The inoculum was prepared from activated sludge taken from Klagshamm waste water plant, Sweden. The activated sludge contained 5.9 g suspended solids /l.
Duration of test (contact time):
35 d
Parameter:
% degradation (DOC removal)
Value:
78
Sampling time:
28 d
Parameter:
% degradation (DOC removal)
Value:
80
Sampling time:
28 d
Details on results:
The biodegradation test was prolongued to 35 days and after this time showed a plateau of approximately 89 % degradation.
The study report gives that the reference compound after 14 days was degraded more than 90 % without giving the reference substance identity.
Interpretation of results:
readily biodegradable, but failing 10-day window
Conclusions:
Biodegradation was determined according to OECD method 301A and is given as 78 and 80 % after 28 days, however, failing the 10 -day window. Thus, the substance is regarded as not ready biodegradable.
Executive summary:

Biodegradation was determined according to OECD method 301A and is given as 78 % and 80 % after 28 days, however, failing the 10 -day window. Thus, the substance is regarded as not ready biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study without detailed documentation.
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 20 +/- 1 °C
Parameter:
% degradation (O2 consumption)
Value:
6
Sampling time:
4 d
Parameter:
% degradation (O2 consumption)
Value:
49
Sampling time:
12 d
Parameter:
% degradation (O2 consumption)
Value:
61
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
80
Sampling time:
20 d
Parameter:
% degradation (O2 consumption)
Value:
84
Sampling time:
28 d
Details on results:
Kinetic of test substance (in %):
6 after 4 day(s)
49 after 12 day(s)
61 after 14 day(s)
80 after 20 day(s)
84 after 26 day(s)
Results with reference substance:
90% after 28 days (Anilin)
Interpretation of results:
readily biodegradable
Conclusions:
Mineralisation of 84% was observed for TMP propoxylated in a manometric test, hence the test material was considered as readily biodegradable.

Description of key information

The % biodegradability for the substance was 84%  in 26 days and therefore it can be considered as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Biodegradation was determined according to OECD method 301A and in a manometric test. In the OECD301 A test, 78 % and 80 % biodegradation was observed after 28 days, however, failing the 10-day window. Thus, the substance is regarded as not ready biodegradable under the stringent conditions of the OECD 301 A. However, the manometric test is a more realistic simulation and in this test 84% biodegradation was observed. Therefore, the substance can be considered as readily biodegradable.