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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The UVCB substance is slightly toxic to aquatic invertebrates. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
51 mg/L

Additional information

To determine the toxicity of the UVCB substance to aquatic invertebrates, a GLP study according to OECD Guideline 202 using Daphnia magna as test organism was carried out. Based on the nominal concentration (loading rate of the WAF) an EC50 (48 h) 51 mg/L was determined [RCC 2004a].

The measured concentrations of the representative components of the test item were approximately in the same range at all loading rates. However, a clear relationship between loading rates of the WAFs and immobility of the daphnids was determined in this test. Thus, the acute toxicity of the test item to daphnids cannot be clearly attributed to the components analyzed in the WAFs. Possibly, a more water soluble fraction compared to the analysed alkylated diphenylamines could have caused these effects.


For this endpoint an additional study with a UVCB substance of similar composition (EC No 253 -249 -4) which is part of the same read-across group as UVCB substance EC No 270 -128 -1 is available. This test was performed using water accommodated fractions and is of equal reliability as the other test on toxicity to Daphnia. No toxicity was observed resulting in following effect concentrations after 48h: EC50 >100 mg/L) [RCC 2004b].

The results of this test with the similar substance EC no 253 -249 -4) can be used as supporting information. C9 -mono- and dialkylated diphenylamines are acutely not hazardous to Daphnia in the range of solubility. It can be concluded that similar substances for example C8 mono and di-alkylated diphenylamine as present in the UVCB substance are also not hazardous to Daphnia in the solubility range. Slight differences in the substance identity and composition might have caused different effects in the Daphnia toxicity tests.


A screening study with the UVCB substance on toxicity to Daphnia is available (RCC 1998). The Daphnia were only exposed for 24 hours to a limit concentration of 100mg/L (loading rate) of the UVCB substance. Effects were observed and the NOEC and EC50 could not be determined. Due to the insufficient exposure time and concentration range, this test is only regarded as supporting.