Trimethyl borate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study, meeting current standards, acceptable for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Principles of method if other than guideline:

Study performed according to internal company standards (BASF-test) before actual guideline was adopted.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

Molecular formula:

CH4O

IUPAC Name:

methanol

Details on test material:

- Name of test material (as cited in study report): methanol
- Analytical purity: 99.8% (Merck)

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: WIGA, laboratory animal breeding, Sulzfeld, Germany)
- Weight at study initiation: 185 ± 85 g
- Diet (e.g. ad libitum): Herilan MRH (H. Eggersmann KG, Rinteln, Germany)
- Water (e.g. ad libitum): tap water


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2 °C
- Humidity (%): 55 ± 5 %
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

nose/head only

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: nose-head inhalation system

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

analytical / nominal concentration:
87.6 / 127 mg/L
115.9 / 159 mg/L
139.0 / 168 mg/L
150.9 /193 mg/L
151.1 / 242 mg/L

No. of animals per sex per dose:

10

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical signs: daily; weighing: before inhalation exposure, 7 and 14 days after inhalation exposure
- Necropsy of survivors performed: yes

Statistics:

probitanalysis [D.J. Finney, 1971, published by the Syndics of the Cambrigdge University Press]

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

128.2 mg/L air

Exp. duration:

4 h

Effect levels 2

Sex:

male

Dose descriptor:

LC50

Effect level:

130.7 mg/L air

Exp. duration:

4 h

Effect levels 3

Sex:

female

Dose descriptor:

LC50

Effect level:

> 115.9 mg/L air

Exp. duration:

4 h

Mortality:

At 87.6 mg/L (analytical value) methanol, no mortality was observed.
Mortally contamined animals died within 5 days after substance administration.

Clinical signs:

other: Mainly in the high concentrations (115.9 - 151.1 mg/L, analytical), where also mortability occurred following clinical symptoms were described: aqueous secretion of eyes and nose, closed eyes, laboring breathing, stagger, apathy, narcosis, prone position,

Body weight:

At concentrations of 150.9 mg/L methanol (analytical value), the body weight of the male animals was reduced at 33 %.

Gross pathology:

Necropsy of deceased animals showed cardiac dilatation and hyperaemic congestion as well as pulmonary edema with extended or scattered hyperaemia. Necropsy of sacrificed animals showed no findings.

Any other information on results incl. tables

At 87.6 mg/L (analytical value) methanol, no mortality was observed.

concentration [mg/L]	lethality (died animals/exposed animals)	lethality (died animals/exposed animals)
analytical	male	female
151.1	10/10	10/10
150.9	8/10	10/10
139.0	6/10	10/10
115.9	2/10	1/10