Registration Dossier

Administrative data

Endpoint:
long-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
Compound: Anethole
CAS: 104-46-1
Purity: 98.9 %
Origin: All test compounds were obtained from Tokyo Kasei Kogyo Co., Tokyo

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
flow-through
Water media type:
other: tap water
Limit test:
yes
Total exposure duration:
28 d

Test conditions

Test temperature:
26  1.5 °C
pH:
7
Nominal and measured concentrations:
0.2 / 1 / 5 mg/L
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
ca. 0.42 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
ca. 2.18 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
ca. 4.14 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
At the highest concentration 5 mg/L, only a few larvae hatched. A few days later, all larvea were dead in all replicates of this concentration.
Therefore, no further analytical measurement was performed at this concentration.
A clear toxicity was also observed at a concentration of 1 mg/L. Only a few surviving larvae are still present at this time.
At the beginning and on day 7 samples were taken from the aquarium. On day 7 the measured concentrations were much too low.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
E-anethole is classified as Long-term (chronic) aquatic hazard Category Chronic 2: