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EC number: 614-925-8 | CAS number: 69098-08-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted in accordance with GLP; low purity of the test substance (72.1 %)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- methyl 3-methoxy-3-(4-methoxyphenyl)propanoate
- EC Number:
- 614-925-8
- Cas Number:
- 69098-08-4
- Molecular formula:
- C12 H16 O4
- IUPAC Name:
- methyl 3-methoxy-3-(4-methoxyphenyl)propanoate
- Details on test material:
- - Name of test material (as cited in study report): 3-(4-Methoxyphenyl)-3-methoxypropionsaueremethylester, technical grade
- Substance No.: 93/98
- Batch No.: Ansatz 165
- Purity: 71.2 %.
- Date of manufacturing: 22 Apr 1993
- Physical state: solid, brown; after heating at about30 °C: liquid brown
- Storage condition of test material: REFRIGERATOR, EXCLUSION OF LIGHT AND AIR (UNDER NITROGEN), EXCLUSION OF MOISTURE
Constituent 1
Method
- Target gene:
- Histidine operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S9-mix
- Test concentrations with justification for top dose:
- - 1st experiment: 20, 100, 500, 2500, 5000 µg/plate
- 2nd, 3rd, 4th and 5th experiment: 1000, 2000, 3000, 4000, 5000 µg/plate - Vehicle / solvent:
- Vehicle used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: With S9-mix: 2.5 µg/plate 2-aminoanthracene for the strains TA 100, TA 98, TA 1537 and TA 1535; without S9-mix: 5 µg/plate N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) for the strains TA 100 and TA 1535, 10 µg/plate 4-nitro-o-phenylenediamine (NPD) for
- Remarks:
- all positive control substances dissolved in DMSO
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
- standard plate test
DURATION
- Preincubation period: no
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3
USED STRAINS
1st experiment: strains TA 1535, TA 100, TA 1537, TA 98
2nd, 3rd, 4th and 5th experiments: strain TA1537
DETERMINATION OF CYTOTOXICITY
- decrease in number of his-revertants
- reduction of titer determination; titer only determined in the experiments with S-9 mix both without test substance (vehicle only) and after adding the two highest doses of test substance
- Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: S. typhimurium TA, 1535, TA 98, TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 2500 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- ambiguous
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 2500 µg/plate depending on the experiment
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: strain/cell type: S. typhimurium TA, 1535, TA 98, TA 100
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Cytotoxicity: A weakly bacteriotoxic effect (slight decrease in the number of his + revertants and a reduced titer) was observed
depending on the strain and test conditions at doses >= 2500 µg/plate.
Mutagenicity:
Tests without S-9 mix:
The strains TA 1535, TA 100 and TA 98 showed no increase in the number of his+ revertants.
The strain TA 1537 showed occasionally some increased colony numbers in the 1st (factor 0.6 -1.5), 3rd (factor 0.5 -1.6),
4th (0.7 - 1.5) and 5th (factor 0.7 -1.4) experiment. The evaluation criteria indicated a positive reaction, when among other things a
doubling of the spontaneous mutation rate was observed. So the findings were stated as not relevant. A positive reaction was
observed in the 2nd experiment from 1000 - 5000 µg/plate (factor 1.8 - 4.4).
Tests with S-9 mix:
The strains TA 1535, TA 100 and TA 98 showed no increase in the number of his+ revertants.
The strain TA 1537 showed no increase in the number of his+ revertants in the 3rd and 4th experiments. Some not relevant, slightly
increased colony numbers were observed in the 1st experiment (factor 1.0 -1.9). A positive reaction was observed in the 2nd
experiment at 2000 - 4000 µg (factor 2.1 - 3.4) and in the 5th experiment at 3000 - 5000 µg/plate (factor 1.9 -3.1).
The positive controls showed the expected increase in his+ revertants/plate.
Results:
Dose µg/plate | metabolic activation | TA98 | TA100 | TA1535 | TA1537 | ||||||||
Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | ||
0 | + | 44 | 34 | 47 | 144 | 107 | 114 | 13 | 11 | 17 | 10 | 10 | 13 |
20 | + | 51 | 37 | 53 | 112 | 121 | 128 | 13 | 20 | 15 | 10 | 11 | 11 |
100 | + | 43 | 46 | 43 | 125 | 138 | 136 | 15 | 16 | 15 | 17 | 7 | 10 |
500 | + | 37 | 41 | 37 | 132 | 127 | 127 | 20 | 14 | 14 | 19 | 10 | 20 |
2500 | + | 39 | 37 | 35 | 134 | 125 | 120 | 13 | 15 | 17 | 19 | 23 | 20 |
5000 | + | 25 | 19 | 25 | 117 | 118 | 132 | 20 | 23 | 15 | 19 | 16 | 16 |
2.5 µg 2-Aminoanthracene | + | 670 | 586 | 676 | 970 | 970 | 930 | 111 | 88 | 96 | 82 | 92 | 114 |
0 | - | 38 | 29 | 39 | 114 | 115 | 89 | 16 | 19 | 23 | 13 | 12 | 14 |
20 | - | 21 | 21 | 26 | 107 | 113 | 122 | 20 | 20 | 20 | 6 | 8 | 8 |
100 | - | 43 | 28 | 30 | 100 | 148 | 121 | 27 | 20 | 21 | 6 | 10 | 9 |
500 | - | 29 | 32 | 23 | 118 | 113 | 116 | 23 | 26 | 21 | 20 | 10 | 9 |
2500 | - | 27 | 39 | 30 | 108 | 128 | 121 | 25 | 22 | 30 | 20 | 14 | 23 |
5000 | - | 42 | 36 | 31 | 117 | 71 | 80 | 29 | 33 | 26 | 18 | 19 | 19 |
5 µg MNNG | - | 873 | 820 | 770 | 920 | 865 | 660 | ||||||
10 µg 4-Nitro-o-phenylendiamin | - | 1054 | 1074 | 982 | |||||||||
100 µg 9-Aminoacridiniumchloride monohydrate | - | 602 | 804 | 843 |
Dose µg/plate | metabolic activation | TA1537 | TA1537 | TA1537 | TA1537 | ||||||||
Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | ||
0 | + | 12 | 16 | 18 | 21 | 8 | 12 | 20 | 15 | 10 | 9 | 10 | 12 |
20 | + | 13 | 10 | 22 | 3 | 15 | 10 | 11 | 15 | 14 | 7 | 12 | 15 |
100 | + | 30 | 33 | 33 | 22 | 12 | 16 | 15 | 7 | 12 | 15 | 6 | 15 |
500 | + | 46 | 43 | 52 | 21 | 11 | 10 | 7 | 12 | 10 | 23 | 12 | 25 |
2500 | + | 47 | 51 | 60 | 13 | 12 | 10 | 12 | 10 | 15 | 29 | 20 | 15 |
5000 | + | 19B | 25B | 30B | 2B | 1B | B | 24 | 12 | 18 | 29 | 28 | 38 |
2.5 µg 2-Aminoanthracene | + | 87 | 87 | 104 | 78 | 89 | 92 | 105 | 110 | 100 | 95 | 101 | 112 |
0 | - | 10 | 10 | 12 | 12 | 6 | 14 | 11 | 12 | 15 | 10 | 8 | 12 |
20 | - | 22 | 17 | 20 | 18 | 6 | 9 | 14 | 14 | 10 | 11 | 8 | 16 |
100 | - | 21 | 36 | 48 | 17 | 15 | 15 | 14 | 4 | 13 | 8 | 10 | 11 |
500 | - | 49 | 55 | 37 | 15 | 16 | 20 | 9 | 19 | 10 | 10 | 8 | 5 |
2500 | - | 34 | 39 | 48 | 10 | 14 | 11 | 16 | 4 | 7 | 4 | 6 | 10 |
5000 | - | 33 | 42 | 33 | 6B | 4B | B | 26 | 18 | 14 | 8 | 16 | 18 |
100 µg 9-Aminoacridiniumchloride monohydrate | - | 418 | 390 | 523 | 490 | 533 | 716 | 659 | 512 | 709 | 342 | 264 | 253 |
B: reduced backround
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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