5,5'-(1H-isoindole-1,3(2H)-diylidene)dibarbituric acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test organisms (species):

Daphnia magna

Details on test organisms:

- Test organism: Daphnia magna STRAUS (clone 5)
- Origin: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Feeding: at least 5 times per week ad libitum with a mix of uicellular green algae
- Culture medium: Elendt M4

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

pH:

7.49 - 7.73

Dissolved oxygen:

6.78 - 8.23

Nominal and measured concentrations:

- Limit test concentration: 2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessels: glass beakers (50 ml volume nominal)
- Fill volume: 20 ml
- Dilution water: prepared according to Directive 92/69/EEC Method C.2, Annex 1
- No. of organisms per vessel: 5
- No. of vessels per concentration and control: 4
- No. of study organisms: 20

FURTHER CONDITIONS
- Illumination: diffuse light, illumination strength max. 20 µE*m-2*s-1
- Photoperiod: 16/h light/dark cycle
- Feeding: Daphnids were not fed during study
- Age of Daphnids: 2 - 24 h old
- Cluture medium: Elendt M4

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

24 h EC50 1.5 mg/L nominal test mat. mobility

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test solutions were prepared according to OECD guideline 23 by mixing 100.1 mg of test substance with 1 l test medium (M4). The mixture was placed in an ultrasonic bath for 5 minutes then stirred on a magnetic stir plate for about 3 days at 20 ± 2 °C to generate a
saturated test solution. Undissolved test substance was removed by filtration with a membrane filter (pore width 0.2 μm).

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): < 24 h

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

2.20 – 3.20 mmol/l

Test temperature:

19.3 – 20.0 °C

pH:

7.8 - 8.1

Dissolved oxygen:

8.1 - 8.9 mg/l

Nominal and measured concentrations:

Nominal test concentrations:
100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes ( 20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic freshwater was used (Elendt M4).
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 μS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: About 20 – 850 lux at a wave length of 400 -750 nm;

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Description of key information

With high probability acutely not harmful to aquatic invertebrates.

Additional information

One guideline study on the toxicity of the substance to Daphnia magna is available. In a static test system set up according to OECD guideline 202 an EC50 > 100 mg/l was determined after 48 hours (BASF SE, 2008).