2,3,4-trichlorobut-1-ene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline test, basic data given

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Reference 2

Reference Type:

secondary source

Title:

2,3,4-TRICHLORO-1-BUTENE

Author:

ECB

Year:

2000

Bibliographic source:

ECB IUCLID dataset

Reference 3

Reference Type:

secondary source

Title:

2,3,4-TRICHLORO-1-BUTENE - CAS No: 2431-50-7

Author:

OECD

Year:

1993

Bibliographic source:

Screening Information Data Set (SIDS) of OECD High Production Volume Chemicals Programme, (1993)

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Test Env. Federal Agency (UBA) 1984. Static test

Principles of method if other than guideline:

Daphnien-Schwimmunfaehigkeits-Test, UBA-Verfahrensvorschlag Mai 1984, Bestimmung der Schwimmunfaehigkeit beim Wasserfloh Daphnia magna, EC0, EC50, EC100 24h, static

GLP compliance:

yes

Remarks:

but no certificate available

Test material

Test material information

Constituent 1

Reference substance name:

2,3,4-trichlorobut-1-ene

EC Number:

219-397-9

EC Name:

2,3,4-trichlorobut-1-ene

Cas Number:

2431-50-7

Molecular formula:

C4H5Cl3

IUPAC Name:

2,3,4-trichlorobut-1-ene

Details on test material:

- Name of test material : 2,3,4-Trichlorbuten-1
- Analytical purity: 94% (cited in ECB IUCLUID)

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the stock solution, 200 mg/L was weighed into water and stirred on a magnetic stirrer for three hours. The solution was kept in movement while drawing off the quantities necessary to produce the test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Waterflea
- Strain: Daphnia magna Straus
- Source: Original from German health authority (BGA), Berlin, than breed in laboratory of Bayer AG, Leverkusen
- Age at study initiation : 6-24 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Nominal and measured concentrations:

Nominal concentrations: Control, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 5.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: cylindrical beaker (diameter 4 cm, high: 6.5 cm), 20 ml test medium
- Aeration: no

- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered tapwater (gravel-pit Mohnheim)


EFFECT PARAMETERS MEASURED: mobility after 24 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

71 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC0=2.0 mg/L (potassium dichromate)