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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The acute immobilisation (EC50) of the test item to Daphnia magna (STRAUS) was determined according to OECD Guideline 202 I/EEC Directive 92/69/EEC Method C.2. The EC50 at 24 h was 63 mg/L and the EC50 at 48 h was 31 mg/L. 

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
31 mg/L

Additional information

The acute immobilisation (EC50) of the test item to Daphnia magna (STRAUS) was determined according to OECD Guideline 202 I/EEC Directive 92/69/EEC Method C.2.

The study was conducted under static conditions over a duration of 48 h. Twenty organisms were exposed to each test concentration and control. The test item was clearly dissolved after filtration of the saturated stock solution in all tested concentration levels throughout exposure. The real test concentrations were calculated based on DOC-analysis:

3.4- 8.0- 16- 35- 75 mg/L.

The EC10and EC50were calculated by probit analysis in the tested concentration range.

EC10-, EC50- (with confidence interval), EC100- values

 

Test duration

[h]

Concentration

[mg/L]

Confidence interval

P= 95 %

EC10

24

25

-

48

17

-

EC50

24

62

50- 70

48

31

27- 36

EC100

24

>75

-

48

>75

-

A reference is carried out once per month with potassium dichromate as reference item and the reference toxicity determined. The EC50value of the reference item at 1.9 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30.

Water quality parameters of pH-value and dissolved oxygen concentration measured at 0 and 48 h were determined to be within the acceptable limits.

The validity criteria of the test guideline were fulfilled.