N-methylaniline

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: 2b. Guideline Study with acceptable restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Remarks:

(see below)

Principles of method if other than guideline:

Slight modifications according to Van Leeuwen, C.J., J.L. Maas-Diepeveen, G. Niebeek, W.H.A.Vergouw, P.S. Griffioen and M.W. Luyken, 1985. Aquatic toxicological aspects of dithiocarbamates and related compounds. Short-term tests. Aquat. Toxicol. 7, 145-164.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Aniline

EC Number:

200-539-3

EC Name:

Aniline

Cas Number:

62-53-3

Molecular formula:

C6H7N

IUPAC Name:

aniline

Constituent 2

Reference substance name:

2-methylaniline

IUPAC Name:

2-methylaniline

Constituent 3

Reference substance name:

3-methylaniline

IUPAC Name:

3-methylaniline

Constituent 4

Reference substance name:

4-methylaniline

IUPAC Name:

4-methylaniline

Details on test material:

Aniline: purity = pro analysis; LogPoct=1.03 (0.90); Mol weight= 93.13;Chs= 0.00
2-methylaniline: purity >= 99.5%; LogPoct=1.54; Mol weight= 107.16;Chs= 0.10
3-methylaniline: purity >= 99%; LogPoct=1.54; Mol weight= 107.16;Chs= - 0.07
4-methylaniline: purity > 97%; LogPoct=1.54; Mol weight= 107.16;Chs= -0.17
Chs: Hammett sigma constant, which is dependent on the position of the substituent.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): isopropanol

Test organisms

Test organisms (species):

Chlorella pyrenoidosa

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

94 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Aniline; 95% C.I.:77-114 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

55 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 2-methylaniline; 95% C.I.: 40-259 mg/L

Effect concentrations 3

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

44 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 3-methylaniline: 95% CL: 40-48 mg/L

Effect concentrations 4

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

138 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 4-methylaniline: 95% CL: 130-148 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

All anilines tested were slightly toxic to green algae with EC50 values for growth between 10 and 100 mg/L , except for 4-methylaniline with an EC50 > 100 mg/L.