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EC number: 278-780-9 | CAS number: 77881-13-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- nov 1985-Aug 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well reported GLP study, comparable to guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 17 beta-Cyano-17 alpha-hydroxy-4-androstene-3-one
- IUPAC Name:
- 17 beta-Cyano-17 alpha-hydroxy-4-androstene-3-one
- Details on test material:
- - Name of test material (as cited in study report): ZK 74.804
- Batch No.: 6471
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Duration of treatment / exposure:
- single administration
- Frequency of treatment:
- single administration
- Post exposure period:
- 24, 48 or 72 h (negative control and treatment group)
24 h (positive control)
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1.0, 2.5 or 5.0 g/kg
Basis:
nominal conc.
application volume: 40 ml/kg
- No. of animals per sex per dose:
- 15-20
- Control animals:
- yes, concurrent vehicle
Examinations
- Tissues and cell types examined:
- Erythrocytes; femur bone marrow smears were prepared and stained using May-Gruenwald and Giemsa solutions.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
A slight but statistically significant increases of micronucleated polychromatic erythrocyte counts were seen at the low and high dose groups versus the concurrent negative control at the last sample time (72 h). However, this increase is biologically not relevant because the control values in males are unusually low (0.6 ± 0.6) compared to those of the first and second sample times (1.4± 1.1 and 1.4± 0.6) and moreover, there was no dose response relationship at all. Additionally, the biological fluctuation is also weIl characterized by the fact that the micronucleated normochromatic erythrocyte count in the high dose group at the last sample time was lower than in all vehicle controls and ZK 74.804 groups.
The positive control compound triaziquone proved to be toxic and gave the expected increase in the micronucleated polychromatic cell counts.
Applicant's summary and conclusion
- Executive summary:
To investigate the potential of AD-Cyanhydrin (= ZK 74.804) to induce chromosome breakage or malfunction of the spindle apparatus an mouse micronucleus test was conducted. Male and female NMRI mice were treated once by gavage with 1.0, 2.5 or 5.0 g/kg body weight. Control animals were given the vehicle at 40 ml/kg in the same manner. Triaziquone (0.15 mg/kg; single i.p. treatment) served as positive control. Animals of the control and treatment groups were killed at intervals of 24, 48 and 72 hours after treatment. The positive control animals were killed 24 hours after treatment. Femur bone marrow smears were prepared and stained using May-Gruenwald and Giemsa solutions. The coded slides were examined for the presence of micronuclei in 1000 polychromatic and 1000 normochromatic erythrocytes and for the ratio of polychromatic to normochromatic erythrocytes. The micronucleated cell counts obtained with ZK 74.804 were comparable with the concurrent control values. No bone marrow depression was observed.
Therefore, AD-Cyanhydrin showed no mutagenic potential when administered by gavage up to 5 g/kg in the mouse micronucleus test.
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