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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Reliability rating was 1 because study followed OECD 202 (Daphnia sp Acute immobilization) test guidelines and was carried out under GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report Date:
1991

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): MCP 968
- Physical state: Liquid
- Analytical purity: 100%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
water soluble fraction (WSF) solutions tested

Test conditions

Hardness:
Hardness of reconstituted freshwater : 207 mg/L as CaCO3
Test temperature:
Test temp: 21-22 C
pH:
pH 7.92 -8.08
Dissolved oxygen:
Dissolved oxygen: 7.5 -7.8 mg/L
Nominal and measured concentrations:
Nominal loadings at 0, 87, 520, 1040, 1994 and 5029 mg/L nominal - generated as water soluble fraction (WSF) solutions
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 5 029 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
nominal loading
Basis for effect:
other: immobilization
Remarks on result:
other: WSF
Duration:
48 h
Dose descriptor:
other: NOEL
Effect conc.:
> 5 029 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
nominal loading
Basis for effect:
other: immobilization
Remarks on result:
other: WSF

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance did not cause any acute toxicity (i.e., immobilization or abnormal behavior) to Daphnia magna at the five concentrations tested including the highest 5029 mg/L loading exposure. The 48-hr EL50 value was determined to be >5029 mg/L WSF. The NOEL (no observed effect loading) after 48 hr was found to be > 5029 mg/L WSF based on no immobilization at this loading.
Executive summary:

The acute toxicity of the test material, as measured by immobilization to the water flea, Daphnia magna, was evaluated in freshwater. Water soluble fractions solutions of the test material did not produce any acute toxicity to Daphnia even at the highest nominal loadings of 5029 mg/L under the conditions of this study. It was determined that the 48 hr EL50 value was > 5029 mg/L WSF and the NOEL was > 5029 mg/L WSF since no immobilization and no adverse effects were observed. Hence, the test material is not expected to produce acute toxicity at its maximal water solubility level in the WSF in this test.